CD159a (NKG2A) antibodies, human

CD159a (NKG2A) antibodies, human

Clone: REA110 | Dilution: 1:11
Clone REA110 recognizes CD159a, an inhibitory natural killer (NK) cell receptor. CD159a forms heterodimer with CD94 and contains a C-type lectin ectodomain. Inhibitory signal is transmitted by the heterodimer via immunoreceptor tyrosine-based inhibitory motifs (ITIMs) and upon ligand engagement, ITIMs are phosphorylated and transmit signal through docking of various tyrosine phosphatases. CD159a/CD94 binds non-classical MHC class I protein, HLA-E. Expression of CD159a is found mainly on NK cells and on subsets of CD8
+
cells.
Additional information: Clone REA110 displays negligible binding to Fc receptors.

Alternative Names

KLRC1, CD159a, NKG2, NKG2A

Technical specifications

  • Antigen: CD159a (NKG2A)
  • Clone: REA110
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: KLRC1, CD159a, NKG2, NKG2A
  • Entrez Gene ID: 3821
  • Molecular mass of antigen [kDa]: 26
  • Cross-reactivity:
    chimpanzee (
    Pan troglodytes
    )
    ,
    olive baboon (
    Papio anubis
    )
    ,
    rhesus monkey (
    Macaca mulatta
    )
    ,
    cynomolgus monkey (
    Macaca fascicularis
    )
  • Distribution of antigen: NK cells, T cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, Biotin

REAfinity clone is tested against different known clones, in a blocking experiment, to identify whether they recognize completely overlapping (++), partially overlapping (+), or completely different epitopes (-). In a blocking experiment, cells are incubated with an excess of purified unconjugated REAfinity clone followed by staining with conjugated form of the other known clones. 

Other clonesOverlap in epitope recognition with REA110
131411+
Z199++
  • Selected References

    1. Rueda, C. et al. (2016) Lipopolysaccharide-Induced Chorioamnionitis Promotes IL-1-Dependent Inflammatory FOXP3+ CD4+ T Cells in the Fetal Rhesus Macaque. J. Immunol. 196(9)
    2. Mahaweni, N. et al. (2018) NKG2A Expression Is Not per se Detrimental for the Anti-Multiple Myeloma Activity of Activated Natural Killer Cells in an In Vitro System Mimicking the Tumor Microenvironment. Front Immunol 9
    3. Jacomet, F. et al. (2017)
      The hypothesis of the human iNKT/innate CD8
      +
      T-cell axis applied to cancer: evidence for a deficiency in chronic myeloid leukemia.
      Front Immunol 7: 688
    4. Borrego, F. et al. (2006) The CD94/NKG2 family of receptors: from molecules and cells to clinical relevance. Immunol. Res. 35(3): 263-278
    5. Wolpert, F. et al. (2015) Interferon-β modulates the innate immune response against glioblastoma initiating cells. PLoS One 10(10): e0139603
    6. Veluchamy, J. P. et al. (2017) High-efficiency lysis of cervical cancer by allogeneic NK cells derived from umbilical cord progenitors is independent of HLA status. Cancer Immunol. Immunother. 66: 51-61
    7. Reeves R. K. et al. (2015) Antigen-specific NK cell memory in rhesus macaques. Nat. Immunol. 16: 927-932
    8. Sullivan, L. C. et al. (2007) The heterodimeric assembly of the CD94-NKG2 receptor family and implications for human leukocyte antigen-E recognition. Immunity 27(6): 900-911
    9. Boyington, J. C. et al. (1999) Structure of CD94 reveals a novel C-type lectin fold: implications for the NK cell-associated CD94/NKG2 receptors. Immunity 10(1): 75-82
    10. Rechtien, A. et al. (2017) Systems Vaccinology Identifies an Early Innate Immune Signature as a Correlate of Antibody Responses to the Ebola Vaccine rVSV-ZEBOV. Cell Rep 20(9)
Product options: 2

Product information

Size
Order no.
Price

CD159a (NKG2A)-Biotin, human

  • REA110
  • 1:11
  • For research use only
for 30
tests
(1)
130-098-822
-

CD159a (NKG2A)-Biotin, human

  • REA110
  • 1:11
  • For research use only
for 100
tests
(1)
130-098-819
-

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