CD146 (LSEC) antibodies, mouse

CD146 (LSEC) antibodies, mouse

Clone: REA1064 | Dilution: 1:50
Clone REA1064 recognizes the mouse CD146 antigen, which is expressed on mouse endothelial cells, including liver sinusoidal endothelial cells (LSECs), smooth muscle cells, and the basal membrane. LSECs are microvascular endothelial cells lining the hepatic sinusoidal wall. Their strategic positioning favors a tight interaction with lymphocytes migrating through the liver. They possess a high capacity for antigen uptake and processing. However, in contrast to professional antigen-presenting cells (e.g. dendritic cells), they express only low levels of costimulatory molecules.
Additional information: Clone REA1064 displays negligible binding to Fc receptors.

Alternative Names

MCAM, 1-gicerin, CD149, MUC18, S-endo, s-gicerin, Endo-CAM, Mel-CAM

Technical specifications

  • Antigen: CD146 (LSEC)
  • Clone: REA1064
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control antibodies
  • Alternative names of antigen: MCAM, 1-gicerin, CD149, MUC18, S-endo, s-gicerin, Endo-CAM, Mel-CAM
  • Entrez Gene ID: 84004
  • Molecular mass of antigen [kDa]: 69
  • Distribution of antigen: dendritic cells, endothelial cells, epithelial cells, fibroblasts, T cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, PE, APC, VioBlue, PE-Vio615, PE-Vio770, APC-Vio770, PerCP-Vio700, VioBright 515, Biotin
  • Selected References

    1. Harder et al. (1991) Dissection of murine lymphocyte-endothelial cell interaction mechanisms by SV-40-transformed mouse endothelial cell lines: novel mechanisms mediating basal binding, and alpha 4-integrin-dependent cytokine-induced adhesion. Exp. Cell Res. 197: 259-267
    2. Diehl, L. et al. (2008)
      Tolerogenic maturation of liver sinusoidal endothelial cells promotes B7-homolog 1-dependent CD8
      +
      T cell tolerance.
      Hepatology 47: 296-305