CD14 antibodies, human

CD14 antibodies, human

Clone: REA599 | Dilution: 1:50
Clone REA599 recognizes the human CD14 antigen. CD14 is part of the functional heteromeric LPS receptor complex comprised of at least CD14, TLR4, and MD-2. It up-regulates cell surface molecules, including adhesion molecules. CD14 is strongly expressed on most human monocytes and macrophages in peripheral blood, other bodily fluids, and in various tissues such as lymph nodes and spleen. CD14 is weakly expressed on subpopulations of human neutrophils and myeloid dendritic cells.
Additional information: Clone REA599 displays negligible binding to Fc receptors.

Alternative names

LPS R

Technical specifications

  • Antigen: CD14
  • Clone: REA599
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: LPS R
  • Entrez Gene ID: 929
  • Molecular mass of antigen [kDa]: 37
  • Cross-reactivity:
    cynomolgus monkey (
    Macaca fascicularis
    )
    , dog, goat, cat, rabbit, mink, bovine, pig, sheep
  • Distribution of antigen: monocytes, macrophages, spleen
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, PE, APC, VioBlue, VioGreen, PE-Vio615, PE-Vio770, APC-Vio770, PerCP-Vio700, Biotin

Specificity

In order to compare the epitope specificity of REAfinity Clone REA599 with other known clones, a competition assay was performed.
 Cells were incubated with an excess of purified unconjugated REAfinity Antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.
 

Other clonesOverlap in epitope recognition with REA599
MφP9-
M5E2++
61D3++
TÜK4++
63D3-
  • Selected references

    1. Goyer, S. M. and Ferrero, E. (1987) Biochemical analysis of myeloid antigen and cDNA expression of gp55 (CD14). Oxford, Oxford University Press
    2. Wilson, A. D. et al. (1995)
      Selection of monoclonal antibodies for the identification of lymphocyte surface antigens in the New World primate
      Saguinus oedipus oedipus
      (cotton top tamarin).
      J. Immunol. Methods 178: 195-200
    3. Dzionek, A. et al. (2000) BDCA-2, BDCA-3, BDCA-4: Three markers for distinct subsets of dendritic cells in human peripheral blood. J. Immunol. 165: 6037-6046
    4. Neu, C. et al. (2013) CD14-dependent monocyte isolation enhances phagocytosis of listeria monocytogenes by proinflammatory, GM-CSF-derived macrophages. PLoS One 8(6): e66898
    5. Paccou, J. et al. (2013)
      Determination and modulation of total and surface calcium-sensing receptor expression in monocytes
      in vivo
      and
      in vitro
      .
      PLoS One 8(10): e74800
Product options: 20
130-110-576

CD14-FITC, human

for 30
tests
(1)
-
130-110-518

CD14-FITC, human

for 100
tests
(1)
-
130-110-577

CD14-PE, human

for 30
tests
(1)
-
130-110-519

CD14-PE, human

for 100
tests
(1)
-
130-110-578

CD14-APC, human

for 30
tests
(1)
-
130-110-520

CD14-APC, human

for 100
tests
(1)
-
130-110-582

CD14-VioBlue, human

for 30
tests
(1)
-
130-110-524

CD14-VioBlue, human

for 100
tests
(1)
-
130-110-583

CD14-VioGreen, human

for 30
tests
(1)
-
130-110-525

CD14-VioGreen, human

for 100
tests
(1)
-
130-110-584

CD14-PE-Vio615, human

for 30
tests
(1)
-
130-110-526

CD14-PE-Vio615, human

for 100
tests
(1)
-
130-110-579

CD14-PE-Vio770, human

for 30
tests
(1)
-
130-110-521

CD14-PE-Vio770, human

for 100
tests
(1)
-
130-110-580

CD14-APC-Vio770, human

for 30
tests
(1)
-
130-110-522

CD14-APC-Vio770, human

for 100
tests
(1)
-
130-110-581

CD14-PerCP-Vio700, human

for 30
tests
(1)
-
130-110-523

CD14-PerCP-Vio700, human

for 100
tests
(1)
-
130-110-575

CD14-Biotin, human

for 30
tests
(1)
-
130-110-517

CD14-Biotin, human

for 100
tests
(1)
-