CD127 antibodies, mouse

CD127 antibodies, mouse

Clone: REA680 | Dilution: 1:50
Clone REA680 recognizes the mouse antigen CD127, also known as IL-7 receptor α chain (IL-7Rα). Mouse CD127 is a 60–90 kDa type I transmembrane glycoprotein. It is a component of the high affinity IL-7 receptor (IL-7R) and the receptor for thymic stromal lymphopoietin (TSLP). CD127 is expressed on immature B cells, CD4
-
CD8
-
, CD4
+
, and CD8
+
thymocytes, naive and memory T cells, thymic NK cells, and bone marrow stromal cells. Upon activation of naive T cells, CD127 expression is downregulated. Re-expression of CD127 identifies the effector cells that will differentiate into memory T cells.
Additional information: Clone REA680 displays negligible binding to Fc receptors.

Alternative Names

IL-7R, IL-7Rα, IL-7Ralpha

Technical specifications

  • Antigen: CD127
  • Clone: REA680
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control antibodies
  • Alternative names of antigen: IL-7R, IL-7Rα, IL-7Ralpha
  • Entrez Gene ID: 16197
  • Molecular mass of antigen [kDa]: 50
  • Distribution of antigen: B cells, bone marrow, liver, lymphocytes, monocytes, T cells, thymocytes
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE
  • Selected References

    1. Colpitts, S. L. et al. (2009)
      IL-7 receptor expression provides the potential for long-term survival of both CD62L
      high
      central memory T cells and Tʜ1 effector cells during
      Leishmania major
      infection.
      J. Immunol. 182: 5702-5711
    2. Vosshenrich, C. A. et al. (2006) A thymic pathway of mouse natural killer cell development characterized by expression of GATA-3 and CD127. Nat. Immunol. 7: 1217-1224
    3. Kaech, S. M. et al. (2003) Selective expression of the interleukin 7 receptor identifies effector CD8 T cells that give rise to long-lived memory cells. Nat. Immunol. 4: 1191-1198
    4. Sudo, T. et al. (1993) Expression and function of the interleukin 7 receptor in murine lymphocytes. Proc. Natl. Acad. Sci. U.S.A. 90: 9125-9129
    5. Goodwin, R. G. et al. (1990) Cloning of the human and murine interleukin-7 receptors: demonstration of a soluble form and homology to a new receptor superfamily. Cell 60: 941-951