CD127 antibodies, human

CD127 antibodies, human

Clone: REA614 | Dilution: 1:50
Clone REA614 recognizes the CD127 antigen, which is the α-chain of the interleukin 7 (IL-7) receptor, a type I membrane glycoprotein. Signaling of IL-7 through the IL-7R requires both IL-7Rα and the common cytokine gamma chain (γc). CD127 can be identified on immature B cells through the early pre–B stage, on thymocytes, and on most mature T cells with transient down-regulation upon activation. On regulatory T cells CD127 is absent and its expression is inversely correlated with FoxP3 expression and suppressive function. CD127 is also used by thymic stromal derived lymphopoietin (TSLP) as part of a complex.
Additional information: Clone REA614 displays negligible binding to Fc receptors.

Alternative names

IL-7R, IL-7Rα, IL-17Ralpha, CDW127, ILRA

Technical specifications

  • Antigen: CD127
  • Clone: REA614
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: IL-7R, IL-7Rα, IL-17Ralpha, CDW127, ILRA
  • Entrez Gene ID: 3575
  • Molecular mass of antigen [kDa]: 50
  • Distribution of antigen: T cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: VioBright FITC, PE, APC, PE-Vio770, APC-Vio770, VioBright 515
  • Selected references

    1. Fry, T. J. and Mackall, C. L. (2002) Interleukin-7: from bench to clinic. Blood 99: 3892-3904
    2. Seddiki, N. et al. (2006) Expression of interleukin (IL)-2 and IL-7 receptors discriminates between human regulatory and activated T cells. J. Exp. Med. 203: 1693-1700
    3. Liu, W. et al. (2006)
      CD127 expression inversely correlates with FoxP3 and suppressive function of human CD4
      +
      T reg cells.
      J. Exp. Med. 203: 1701-1711
    4. Sudo, T. et al. (1993) Expression and function of the interleukin 7 receptor in murine lymphocytes. Proc. Natl. Acad. Sci. U.S.A. 90: 9125-9129
    5. Cupedo, T. et al. (2005) Development and activation of regulatory T cells in the human fetus. Eur. J. Immunol. 35: 383-390
    6. Armitage, R. J. et al. (1991) Expression of receptors for interleukin 4 and interleukin 7 on human T cells. Adv. Exp. Med. Biol. 292: 121-130

REAfinity clone is tested against different known clones, in a blocking experiment, to identify whether they recognize completely overlapping (++), partially overlapping (+), or completely different epitopes (-). In a blocking experiment, cells are incubated with an excess of purified unconjugated REAfinity clone followed by staining with conjugated form of the other known clones. 

Other clonesOverlap in epitope recognition with REA614
HIL-7R-M21++
eBioRDR5-
A019D5++
MB15-18C9++
Product options: 12
130-113-979

CD127-VioBright FITC, human

for 30
tests
(1)
-
130-113-417

CD127-VioBright FITC, human

for 100
tests
(1)
-
130-113-976

CD127-PE, human

for 30
tests
(1)
-
130-113-414

CD127-PE, human

for 100
tests
(1)
-
130-113-975

CD127-APC, human

for 30
tests
(1)
-
130-113-413

CD127-APC, human

for 100
tests
(1)
-
130-113-977

CD127-PE-Vio770, human

for 30
tests
(1)
-
130-113-415

CD127-PE-Vio770, human

for 100
tests
(1)
-
130-113-978

CD127-APC-Vio770, human

for 30
tests
(1)
-
130-113-416

CD127-APC-Vio770, human

for 100
tests
(1)
-
130-120-113

CD127-VioBright 515, human

for 30
tests
(1)
-
130-120-027

CD127-VioBright 515, human

for 100
tests
(1)
-