CD127 antibodies, human

CD127 antibodies, human

Clone: REA614 | Dilution: 1:50
Clone REA614 recognizes the CD127 antigen, which is the α-chain of the interleukin 7 (IL-7) receptor, a type I membrane glycoprotein. Signaling of IL-7 through the IL-7R requires both IL-7Rα and the common cytokine gamma chain (γc). CD127 can be identified on immature B cells through the early pre–B stage, on thymocytes, and on most mature T cells with transient down-regulation upon activation. On regulatory T cells CD127 is absent and its expression is inversely correlated with FoxP3 expression and suppressive function. CD127 is also used by thymic stromal derived lymphopoietin (TSLP) as part of a complex.
Additional information: Clone REA614 displays negligible binding to Fc receptors.

Alternative Names

IL-7R, IL-7Rα, IL-17Ralpha, CDW127, ILRA

Technical specifications

  • Antigen: CD127
  • Clone: REA614
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: IL-7R, IL-7Rα, IL-17Ralpha, CDW127, ILRA
  • Entrez Gene ID: 3575
  • Molecular mass of antigen [kDa]: 50
  • Distribution of antigen: T cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: VioBright FITC, PE, APC, PE-Vio770, APC-Vio770
  • Selected References

    1. Fry, T. J. and Mackall, C. L. (2002) Interleukin-7: from bench to clinic. Blood 99: 3892-3904
    2. Seddiki, N. et al. (2006) Expression of interleukin (IL)-2 and IL-7 receptors discriminates between human regulatory and activated T cells. J. Exp. Med. 203: 1693-1700
    3. Liu, W. et al. (2006)
      CD127 expression inversely correlates with FoxP3 and suppressive function of human CD4
      +
      T reg cells.
      J. Exp. Med. 203: 1701-1711
    4. Sudo, T. et al. (1993) Expression and function of the interleukin 7 receptor in murine lymphocytes. Proc. Natl. Acad. Sci. U.S.A. 90: 9125-9129
    5. Cupedo, T. et al. (2005) Development and activation of regulatory T cells in the human fetus. Eur. J. Immunol. 35: 383-390
    6. Armitage, R. J. et al. (1991) Expression of receptors for interleukin 4 and interleukin 7 on human T cells. Adv. Exp. Med. Biol. 292: 121-130

REAfinity clone is tested against different known clones, in a blocking experiment, to identify whether they recognize completely overlapping (++), partially overlapping (+), or completely different epitopes (-). In a blocking experiment, cells are incubated with an excess of purified unconjugated REAfinity clone followed by staining with conjugated form of the other known clones. 

Other clonesOverlap in epitope recognition with REA614
HIL-7R-M21++
eBioRDR5-
A019D5++
MB15-18C9++
Product options: 10

Product information

Size
Order no.
Price

CD127-VioBright FITC, human

  • REA614
  • 1:50
  • For research use only
for 30
tests
(1)
130-113-979
-

CD127-VioBright FITC, human

  • REA614
  • 1:50
  • For research use only
for 100
tests
(1)
130-113-417
-

CD127-PE, human

  • REA614
  • 1:50
  • For research use only
for 30
tests
(1)
130-113-976
-

CD127-PE, human

  • REA614
  • 1:50
  • For research use only
for 100
tests
(1)
130-113-414
-

CD127-APC, human

  • REA614
  • 1:50
  • For research use only
for 30
tests
(1)
130-113-975
-

CD127-APC, human

  • REA614
  • 1:50
  • For research use only
for 100
tests
(1)
130-113-413
-

CD127-PE-Vio770, human

  • REA614
  • 1:50
  • For research use only
for 30
tests
(1)
130-113-977
-

CD127-PE-Vio770, human

  • REA614
  • 1:50
  • For research use only
for 100
tests
(1)
130-113-415
-

CD127-APC-Vio770, human

  • REA614
  • 1:50
  • For research use only
for 30
tests
(1)
130-113-978
-

CD127-APC-Vio770, human

  • REA614
  • 1:50
  • For research use only
for 100
tests
(1)
130-113-416
-