CD127 antibodies – functional grade, mouse

CD127 antibodies – functional grade, mouse

Clone: A7R 34
The antibody clone A7R34 reacts with CD127, also known as interleukin 7 receptor α chain (IL-7Rα). Mouse CD127 is a 60–90 kDa type I transmembrane glycoprotein. It is a component of the high affinity IL-7 receptor (IL-7R) and the receptor for thymic stromal lymphopoietin (TSLP). CD127 is expressed on immature B cells, CD4
-
CD8
-
, CD4
+
, and CD8
+
thymocytes, naive and memory T cells, thymic NK cells, and bone marrow stromal cells. Upon activation of naive T cells, CD127 expression is down-regulated. Re-expression of CD127 identifies the effector cells that will differentiate into memory T cells.

Applications

This pure – functional grade antibody is suited for cell culture functional assays, for example, blocking of receptor-ligand binding.

Alternative names

IL-7R, IL-7Rα

Technical specifications

  • Antigen: CD127
  • Clone: A7R 34
  • Isotype: rat IgG2aκ
  • Alternative names of antigen: IL-7R, IL-7Rα
  • Entrez Gene ID: 16197
  • Molecular mass of antigen [kDa]: 50
  • Distribution of antigen: B cells, bone marrow, liver, lymphocytes, monocytes, T cells, thymocytes
  • Endotoxin level: Endotoxin levels have been tested and do not exceed 0.01 ng/µg of protein.
  • Product format: Antibodies are supplied in phosphate-buffered saline (PBS), pH 7.2. The product contains no preservative and is sterile filtered. Always handle under aseptic conditions.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates:
  • Selected references

    1. Goodwin, R. G. et al. (1990) Cloning of the human and murine interleukin-7 receptors: demonstration of a soluble form and homology to a new receptor superfamily. Cell 60: 941-951
    2. Park, L. S. et al. (2000) Cloning of the murine thymic stromal lymphopoietin (TSLP) receptor: Formation of a functional heteromeric complex requires interleukin 7 receptor. J. Exp. Med. 192(5): 659-670
    3. Sudo, T. et al. (1993) Expression and function of the interleukin 7 receptor in murine lymphocytes. Proc. Natl. Acad. Sci. U.S.A. 90: 9125-9129
    4. Vosshenrich, C. A. et al. (2006) A thymic pathway of mouse natural killer cell development characterized by expression of GATA-3 and CD127. Nat. Immunol. 7: 1217-1224
    5. Kaech, S. M. et al. (2003) Selective expression of the interleukin 7 receptor identifies effector CD8 T cells that give rise to long-lived memory cells. Nat. Immunol. 4: 1191-1198
    6. Colpitts, S. L. et al. (2009)
      IL-7 receptor expression provides the potential for long-term survival of both CD62L
      high
      central memory T cells and Tʜ1 effector cells during
      Leishmania major
      infection.
      J. Immunol. 182: 5702-5711
Product options: 1
130-094-828

CD127 pure - functional grade, mouse

1 mg in 0.5 mL
-