CD125 (IL-5Rα) antibodies, mouse

CD125 (IL-5Rα) antibodies, mouse

Clone: REA343 | Dilution: 1:10
Clone REA343 recognizes the mouse CD125 antigen, a single-pass type I membrane protein, also known as interleukin-5 receptor subunit α (IL-5Rα). The IL-5 receptor is a heterodimer composed of a ligand specific α subunit and a signal transducing β subunit which is shared by the receptors for IL-3, GM-CSF, and IL-5. CD125 is expressed on eosinophils, basophils, and B cells. IL-5 is a conserved hematopoietic cytokine which is mainly produced by Tʜ2 cells after stimulation with antigens, and by mast cells upon stimulation with an allergen/IgE complex or a calcium ionophore. The overexpression of IL-5
in vivo
significantly increases the numbers of eosinophils and B cells, while mice lacking the functional gene for IL-5 display various developmental and functional impairments in the B cell and eosinophil lineages.
Additional information: Clone REA343 displays negligible binding to Fc receptors.

Alternative Names

CDw125, Il5r, IL-5RA

Technical specifications

  • Antigen: CD125 (IL-5Rα)
  • Clone: REA343
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control antibodies
  • Alternative names of antigen: CDw125, Il5r, IL-5RA
  • Entrez Gene ID: 16192
  • Molecular mass of antigen [kDa]: 45
  • Distribution of antigen: granulocytes, basophils, eosinophils, B cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE, APC, PE-Vio770, APC-Vio770, PerCP-Vio700, Biotin
REAfinity clone is tested against different hybridoma clones, in a blocking experiment, to identify whether they recognize completely overlapping (++), partially overlapping (+), or completely different epitopes (-). In a blocking experiment, cells are incubated with an excess of purified unconjugated REAfinity clone followed by staining with conjugated form of the different hybridoma clones. 
Hybridoma cloneOverlap in epitope recognition with REA343
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