Anti-TRA-1-60 Live Cell Stain antibodies, human

Anti-TRA-1-60 Live Cell Stain antibodies, human

Clone: REA157
Anti-TRA-1-60 Live Cell Stain antibodies have been developed for the detection of TRA-1-60
+
cells directly in culture without the need for fixation, e.g., to facilitate colony picking after reprogramming or to evaluate pluripotency of a culture directly in the cell culture dish.
Clone REA157 recognizes TRA-1-60 antigen, a neuraminidase-resistant carbohydrate epitope expressed on podocalyxin, which is a transmembrane glycoprotein. Podocalyxin is a member of the CD34-related family of sialomucins and is recognized as a stem cell and tumour marker. TRA-1-60 antigen is expressed on the surface of human embryonic stem cells (ESC), embryonic germ cells (EG), and embryonic carcinoma cells (EC). Expression of TRA-1-60 is lost upon differentiation and thus it can be used to assess the differentiation stage of the cells. TRA-1-60 can be detected in the serum of patients with germ cell tumors which contain an EC component.
Additional information: Clone REA157 displays negligible binding to Fc receptors.

Alternative Names

PODXL, Gp200, PC, PCLP, PCLP-1, Podocalyxin

Technical specifications

  • Antigen: TRA-1-60
  • Clone: REA157
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: PODXL, Gp200, PC, PCLP, PCLP-1, Podocalyxin
  • Distribution of antigen: ES and iPS cells
  • Endotoxin level: Low endotoxin levels as determined by LAL assay.
  • Product format: Antibodies are supplied in phosphate-buffered saline (PBS), pH 7.2. The product contains no preservative and is sterile filtered. Always handle under aseptic conditions.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: Vio488
  • Selected References

    1. Andrews, P. W. et al. (1984) Three monoclonal antibodies defining distinct differentiation antigens associated with different high molecular weight polypeptides on the surface of human embryonal carcinoma cells. Hybridoma 3(4): 347-361
    2. Lajer, H. et al. (2002) Clinical use of serum TRA-1-60 as tumor marker in patients with germ cell cancer. Int. J. Cancer 100(2): 244-246
    3. Schopperle, W. M. et al. (2007) The TRA-1-60 and TRA-1-81 human pluripotent stem cell markers are expressed on podocalyxin in embryonal carcinoma. Stem Cells 25(3): 723-730
Product options: 1

Product information

Size
Order no.
Price

Anti-TRA-1-60-Vio488 Live Cell Stain, human

  • REA157
  • For research use only
for
up to
10 mL_medium
130-106-872
-