Anti-TNF-α antibodies, mouse

Anti-TNF-α antibodies, mouse

Clone: REA636 | Dilution: 1:50
Clone REA636 recognizes the mouse tumor necrosis factor α (TNF-α) antigen, a 17 kDa cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF-α is produced by cells that are involved in inflammatory immune responses. It is secreted by activated CD4
+
T cells, monocytes, macrophages, NK cells, and neutrophils. TNF-α binding to surface receptors elicits a wide array of biologic activities, including cytolysis and cytostasis of many tumor cell lines
in vitro
, hemorraghic necrosis of tumors
in vivo
, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils. Furthermore it causes fever by direct action or by stimulation of interleukin-1 secretion.
Additional information: Clone REA636 displays negligible binding to Fc receptors.

Alternative Names

TNFa, TNFalpha, TNF-alpha, Cachectin, Tnfsf1a, TNFSF2

Technical specifications

  • Antigen: TNF-α
  • Clone: REA636
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control antibodies
  • Alternative names of antigen: TNFa, TNFalpha, TNF-alpha, Cachectin, Tnfsf1a, TNFSF2
  • Entrez Gene ID: 21926
  • Molecular mass of antigen [kDa]: 26
  • Distribution of antigen: T cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE
  • Selected References

    1. Chyuan, I. T. et al. (2015) Tumor necrosis factor-alpha blockage therapy impairs hepatitis B viral clearance and enhances T-cell exhaustion in a mouse model. Cell. Mol. Immunol. 12(3): 317-325
    2. Torrente, Y. et al. (2003) Tumor necrosis factor-alpha (TNF-alpha) stimulates chemotactic response in mouse myogenic cells. Cell Transplant 12(1): 91-100
    3. Kaiser, G. C. et al. (1997) Tumor necrosis factor alpha regulates proliferation in a mouse intestinal cell line. Gastroenterology 112(4): 1231-1240