Anti-TNF-α antibodies, human

Anti-TNF-α antibodies, human

Clone: REA656 | Dilution: 1:50
Clone REA656 recognizes the human tumor necrosis factor alpha (TNF-α) antigen, which is a cytokine involved in inflammatory immune responses. TNF-α has different functions, it plays an important role in the regulation of immune cells and is a potent pyrogen causing fever and apoptotic cell death. Furthermore, the TNF intracellular domain form is able to induce interleukin 12 (IL-12) production in dendritic cells. TNF-α is secreted by activated CD4
+
T cells, monocytes, macrophages, natural killer (NK) cells, mast cells, eosinophils, and neutrophils.
Additional information: Clone REA656 displays negligible binding to Fc receptors.

Alternative Names

Tumor necrosis factor, Cachectin, TNF-alpha, TNF-a, DIF-alpha, TNFa, TNFSF2

Technical specifications

  • Antigen: TNF-α
  • Clone: REA656
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (I) antibodies
  • Alternative names of antigen: Tumor necrosis factor, Cachectin, TNF-alpha, TNF-a, DIF-alpha, TNFa, TNFSF2
  • Entrez Gene ID: 7124
  • Molecular mass of antigen [kDa]: 26
  • Distribution of antigen: T cells, monocytes, macrophages, NK cells, mast cells, eosinophils, neutrophils
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE, APC

REAfinity clone is tested against different known clones, in a blocking experiment, to identify whether they recognize completely overlapping (++), partially overlapping (+), or completely different epitopes (-). In a blocking experiment, cells are incubated with an excess of purified unconjugated REAfinity clone followed by staining with conjugated form of the other known clones. 

Other clonesOverlap in epitope recognition with REA656
cA2++
mab11++
357-167-24-
  • Selected References

    1. Friedmann, E. et al. (2006) SPPL2a and SPPL2b promote intramembrane proteolysis of TNFalpha in activated dendritic cells to trigger IL-12 production. Nat. Cell Biol. 8(8): 843-848
    2. Nedwin, G. E. et al. (1985) Human lymphotoxin and tumor necrosis factor genes: structure, homology and chromosomal localization. Nucleic Acids Res. 13(17): 6361-6373
    3. Nedospasov, S. A. et al. (1985) Tandem arrangement of genes coding for tumor necrosis factor (TNF-alpha) and lymphotoxin (TNF-beta) in the human genome. Cold Spring Harb. Symp. Quant. Biol. 51(1): 611-624
Product options: 4

Product information

Size
Order no.
Price

Anti-TNF-α-PE, human

  • REA656
  • 1:50
  • For research use only
for 30
tests
(1)
130-119-151
-

Anti-TNF-α-PE, human

  • REA656
  • 1:50
  • For research use only
for 100
tests
(1)
130-118-974
-

Anti-TNF-α-APC, human

  • REA656
  • 1:50
  • For research use only
for 30
tests
(1)
130-120-149
-

Anti-TNF-α-APC, human

  • REA656
  • 1:50
  • For research use only
for 100
tests
(1)
130-120-063
-