Anti-TNF-α antibodies – functional grade, mouse

Anti-TNF-α antibodies – functional grade, mouse

Clone: MP6-XT22
Tumor necrosis factor alpha (TNF-α) is produced by cells that are involved in inflammatory immune responses. TNF-α is secreted by activated CD4
+
T cells, monocytes, macrophages, NK cells, and neutrophils.
Anti-TNF-α antibodies have been designed for intracellular staining of TNF-α–producing cells. Cells can be stimulated for TNF-α production, for example, by polyclonal stimulation with mitogens. For induction of TNF-α production by antigen-specific T cells, cells are restimulated with the respective antigen. TNF-α can be accumulated in the cells by addition of secretion inhibitors like brefeldin A. After fixation and permeabilization of the cell sample, TNF-α–producing cells can be stained intracellularly with Anti-TNF-α antibodies. Staining for surface markers allows simultaneous flow cytometric analysis of subsets and activation status of the TNF-α–producing cells.

Applications

The Anti-TNF-α pure – functional grade antibody is suited for functional grade assays, for example, neutralization of TNF-α activity.

Alternative Names

TNF, DIF, TNFSF2, Tnfsf1a

Technical specifications

  • Antigen: TNF-α
  • Clone: MP6-XT22
  • Isotype: rat IgG1
  • Alternative names of antigen: TNF, DIF, TNFSF2, Tnfsf1a
  • Entrez Gene ID: 21926
  • Molecular mass of antigen [kDa]: 26
  • Endotoxin level: Endotoxin levels have been tested and do not exceed 0.01 ng/µg of protein.
  • Product format: Antibodies are supplied in phosphate-buffered saline (PBS), pH 7.2. The product contains no preservative and is sterile filtered. Always handle under aseptic conditions.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: pure
Product options: 1

Product information

Size
Order no.
Price

Anti-TNF-α pure - functional grade, mouse

  • MP6-XT22
  • For research use only
500 µg in 0.5 mL
130-095-725
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