Anti-TIM-3 antibodies, mouse

Anti-TIM-3 antibodies, mouse

Clone: REA602 | Dilution: 1:50
Clone REA602 reacts with the mouse TIM-3 antigen, which is a Tʜ1-specific cell surface protein expressed by differentiated CD4
+
Tʜ1 and CD8
+
Tc1 cells and macrophages. It is absent on CD4
+
Tʜ2 and CD8
+
Tc2 cells. TIM-3 has been proposed to inhibit Tʜ1-mediated immune responses. TIM-3 absence enhances the severity of experimental autoimmune encephalomyelitis in mice. The interaction of TIM-3 and galectin-9 induces apoptosis of Tʜ1 cells.
Additional information: Clone REA602 displays negligible binding to Fc receptors.

Alternative names

Tim3

Technical specifications

  • Antigen: TIM-3
  • Clone: REA602
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control antibodies
  • Alternative names of antigen: Tim3
  • Entrez Gene ID: 171285
  • Molecular mass of antigen [kDa]: 29
  • Distribution of antigen: macrophages
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE, APC, VioBright FITC
  • Selected references

    1. Monney, L. et al. (2002) Tʜ1-specific cell surface protein Tim-3 regulates macrophage activation and severity of an autoimmune disease. Nature 415: 536-541
    2. van de Weyer, P. S. et al. (2006) A highly conserved tyrosine of Tim-3 is phosphorylated upon stimulation by its ligand galectin-9. Biochem. Biophys. Res. Commun. 351(2): 571-576
  • Customer reports and application notes

Product options: 5
130-118-691

Anti-TIM-3-PE, mouse

30 µg in 200 µL (1)
-
130-118-563

Anti-TIM-3-PE, mouse

150 µg in 1 mL (1)
-
130-119-796

Anti-TIM-3-APC, mouse

30 µg in 200 µL (1)
-
130-119-756

Anti-TIM-3-APC, mouse

150 µg in 1 mL (1)
-
130-120-823

Anti-TIM-3-VioBright FITC, mouse

30 µg in 200 µL (1)
-