Anti-TCRγ/δ antibodies, human

Anti-TCRγ/δ antibodies, human

Clone: REA591 | Dilution: 1:50
Clone REA591 recognizes the human TCRγ/δ antigen. The T cell receptor is a heterodimeric glycoprotein associated with the CD3 antigen. It consists of an α and a β chain (TCRα/β) or a γ and a δ chain (TCRγ/δ). The γ and δ TCR chains are composed of constant and variable regions, each encoded by distinct gene segments. The γ chain forms either disulfide-linked or non-disulfide–linked heterodimers with the δ-subunit. The γ/δ T cell receptor is present on a subset of T lymphocytes in peripheral blood. TCRγ/δ is involved in the antigen recognition of tumor-associated antigens or bacterial antigens presented by MHC class I molecules.
Additional information: Clone REA591 displays negligible binding to Fc receptors.

Alternative Names

TCRgd

Technical specifications

  • Antigen: TCRγ/δ
  • Clone: REA591
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: TCRgd
  • Entrez Gene ID: 6965
  • Distribution of antigen: T cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, PE, APC, PE-Vio770, APC-Vio770, PerCP-Vio700, Biotin

Specificity

In order to compare the epitope specificity of REAfinity Clone REA591 with other known clones, a competition assay was performed.
 Cells were incubated with an excess of purified unconjugated REAfinity Antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.
 

Other clonesOverlap in epitope recognition with REA591
B1+
11F2++
B1.1+
IMMU510++
  • Selected References

    1. Airoldi, I. et al. (2015)
      γδ T-cell reconstitution after HLA-haploidentical hematopoietic transplantation depleted of TCR-αβ
      +
      /CD19
      +
      lymphocytes.
      Blood 125(15): 2349-2358
    2. Bonneville, M. et al. (2010) Gammadelta T cell effector functions: a blend of innate programming and acquired plasticity. Nat. Rev. Immunol. 10(7): 467-478
    3. Wiest, D. L. (2016) Development of γδ T cells, the special-force soldiers of the immune system. Methods Mol. Biol. 1323: 23-32
Product options: 14
130-114-037

Anti-TCRγ/δ-FITC, human

for 30
tests
(1)
-
130-113-511

Anti-TCRγ/δ-FITC, human

for 100
tests
(1)
-
130-114-038

Anti-TCRγ/δ-PE, human

for 30
tests
(1)
-
130-113-512

Anti-TCRγ/δ-PE, human

for 100
tests
(1)
-
130-114-034

Anti-TCRγ/δ-APC, human

for 30
tests
(1)
-
130-113-508

Anti-TCRγ/δ-APC, human

for 100
tests
(1)
-
130-114-039

Anti-TCRγ/δ-PE-Vio770, human

for 30
tests
(1)
-
130-113-513

Anti-TCRγ/δ-PE-Vio770, human

for 100
tests
(1)
-
130-114-035

Anti-TCRγ/δ-APC-Vio770, human

for 30
tests
(1)
-
130-113-509

Anti-TCRγ/δ-APC-Vio770, human

for 100
tests
(1)
-
130-114-040

Anti-TCRγ/δ-PerCP-Vio700, human

for 30
tests
(1)
-
130-113-514

Anti-TCRγ/δ-PerCP-Vio700, human

for 100
tests
(1)
-
130-114-036

Anti-TCRγ/δ-Biotin, human

for 30
tests
(1)
-
130-113-510

Anti-TCRγ/δ-Biotin, human

for 100
tests
(1)
-