Anti-TCRβ antibodies, mouse

Anti-TCRβ antibodies, mouse

Clone: REA318 | Dilution: 1:50
Clone REA318 recognizes the mouse T cell receptor beta chain (TCRβ). The T cell receptor (TCR) is responsible for recognizing antigens bound to major histocompatibility complex (MHC) molecules. It is a disulfide-linked membrane-anchored heterodimeric glycoprotein normally consisting of the highly variable alpha and beta chains expressed as part of a complex with the invariant CD3 chain molecules. T cells expressing this receptor are referred to as TCRα/β T cells, though a minority of T cells express an alternate receptor, formed by variable gamma and delta chains, referred as TCRγ/δ T cells. TCRβ is expressed on TCRα/β T cells and thymocytes, NK1.1
+
thymocytes and NK-T cells.
Additional information: Clone REA318 displays negligible binding to Fc receptors.

Alternative Names

TCRB, TCRbeta, Tib

Technical specifications

  • Antigen: TCRβ
  • Clone: REA318
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control antibodies
  • Alternative names of antigen: TCRB, TCRbeta, Tib
  • Entrez Gene ID: 21577
  • Distribution of antigen: T cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: APC-Vio770

REAfinity clone is tested against different known clones, in a blocking experiment, to identify whether they recognize completely overlapping (++), partially overlapping (+), or completely different epitopes (-). In a blocking experiment, cells are incubated with an excess of purified unconjugated REAfinity clone followed by staining with conjugated form of the other known clones. 

Other clonesOverlap in epitope recognition with REA318
H57-597++
  • Selected References

    1. Robins, H.S. et al. (2009) Comprehensive assessment of T-cell receptor β-chain diversity in αβ T cells. Blood 114(19): 4099-4107
    2. Schumm, M. et al. (2013) Depletion of T-cell receptor alpha/beta and CD19 positive cells from apheresis products with the CliniMACS device. Cytotherapy 15(10): 1253-1258
    3. Okuno, Y. et al. (2013)
      CD8
      +
      CD122
      +
      regulatory T cells contain clonally expanded cells with identical CDR3 sequences of the T-cell receptor β-chain.
      Immunology 139(3): 309-317
Product options: 2

Product information

Size
Order no.
Price

Anti-TCRβ-APC-Vio770, mouse

  • REA318
  • 1:50
  • For research use only
30 µg in 200 µL (1)
130-120-289
-

Anti-TCRβ-APC-Vio770, mouse

  • REA318
  • 1:50
  • For research use only
150 µg in 1 mL (1)
130-120-215
-