Anti-T-bet antibodies, human and mouse

Anti-T-bet antibodies, human and mouse

Clone: REA102 | Dilution: 1:50
The monoclonal antibody REA102 recognizes human and mouse T-bet, a Tʜ1-specific T-box 1 transcription factor. T-bet is considered to be an important regulator of Tʜ1 lymphoid development and is involved in class-switch recombination in B cells. It is widely expressed in hematopoietic cells such as B cells, T cell subsets, NK and NKT cells, and dendritic cells. Expression of T-bet is induced by Tʜ1 cytokine IFN-γ and T-bet further regulates the expression of cytokines including IFN-γ, IL-12Rβ, and IL-2.
Additional information: Clone REA102 displays negligible binding to Fc receptors.

Alternative Names

TBX21, T-PET, TBLYM, TBT1

Technical specifications

  • Antigen: T-bet
  • Clone: REA102
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (I) antibodies
  • Alternative names of antigen: TBX21, T-PET, TBLYM, TBT1
  • Entrez Gene ID: 30009
  • Molecular mass of antigen [kDa]: 58
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: APC
  • Selected References

    1. Szabo, S. J. et al. (2002) Distinct effects of T-bet in Th1 lineage commitment and IFN-gamma production in CD4 and CD8 T cells. Science 295: 338
    2. Lugo-Villarino, G. et al. (2003) T-bet is required for optimal production of IFN-γ and antigen-specific T cell activation by dendritic cells. Proc. Natl. Acad. Sci. U.S.A. 100: 7749-7754
    3. Peng, S. L. et al. (2002) T-bet regulates IgG class switching and pathogenic autoantibody production. Proc. Natl. Acad. Sci. U.S.A. 99: 5545-5550
    4. Szabo, S. J. et al. (2000) A novel transcription factor, T-bet, directs Tʜ1 lineage commitment. Cell 100(6): 655-669
    5. Qian, L. et al. (2014) Alkylglycerols modulate the proliferation and differentiation of non-specific agonist and specific antigen-stimulated splenic lymphocytes. PLoS One 9(4): e96207