Anti-STAT1 pS727 antibodies, human

Anti-STAT1 pS727 antibodies, human

Clone: REA525 | Dilution: 1:11
Clone REA525 recognizes the signal transducer and activator of transcription 1 (STAT1) antigen phosphorylated at serine 727 (pS727). STAT1 expression is found ubiquitously. It is involved in upregulating genes due to a signal by either type I, type II, or type III interferons. STAT1 is an essential transcription factor for macrophage activation by IFN-γ and requires phosphorylation of the C-terminal pS727 for transcriptional activity. It forms homodimers or heterodimers with STAT3 that bind to the IFN-γ activated sequence promoter element. In response to either IFN-α or IFN-β stimulation, STAT1 forms a heterodimer with STAT2 that can bind the interferon stimulated response promoter element. In either case, binding of the promoter element leads to an increased expression of interferon stimulated genes.
Additional information: Clone REA525 displays negligible binding to Fc receptors.

Alternative names

CANDF7, IMD31A, IMD31B, IMD31C, ISGF-3, STAT91

Technical specifications

  • Antigen: STAT1 pS727
  • Clone: REA525
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (I) antibodies
  • Alternative names of antigen: CANDF7, IMD31A, IMD31B, IMD31C, ISGF-3, STAT91
  • Entrez Gene ID: 6772
  • Molecular mass of antigen [kDa]: 87
  • Distribution of antigen: ubiquitous
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, PE, APC
  • Selected references

    1. Kovarik, P. et al. (1999) Stress-induced phosphorylation of STAT1 at Ser727 requires p38 mitogen-activated protein kinase whereas IFN-gamma uses a different signaling pathway. Proc. Natl. Acad. Sci. U.S.A. 96(24): 13956-13961
    2. Vanhatupa, S. et al. (2008) MAPK-induced Ser727 phosphorylation promotes SUMOylation of STAT1. Biochem. J. 409(1): 179-185
    3. Luu, K. et al. (2014) STAT1 plays a role in TLR signal transduction and inflammatory responses. Immunol. Cell Biol. 92(9): 761-769
Product options: 6
130-107-987

Anti-STAT1 pS727-FITC, human

for 30
tests
(1)
-
130-107-925

Anti-STAT1 pS727-FITC, human

for 100
tests
(1)
-
130-107-988

Anti-STAT1 pS727-PE, human

for 30
tests
(1)
-
130-107-926

Anti-STAT1 pS727-PE, human

for 100
tests
(1)
-
130-107-989

Anti-STAT1 pS727-APC, human

for 30
tests
(1)
-
130-107-927

Anti-STAT1 pS727-APC, human

for 100
tests
(1)
-