Anti-SSEA-1 antibodies, human and mouse

Anti-SSEA-1 antibodies, human and mouse

Clone: REA321 | Dilution: 1:50
Clone REA321 recognizes the human and mouse stage specific embryonic antigen 1 (SSEA-1) antigen, which is a Lewis blood group related carbohydrate antigen, also known as CD15. SSEA-1 is expressed on human myelomonocytic cells and is present on neutrophils, eosinophils, and some monocytes, but not on basophils or lymphocytes. The antigen is also expressed on Hodgkin/Reed-Sternberg cells. SSEA-1 is a common surface marker for pluripotency on mouse ES and iPS cells. On human cells in contrast, SSEA-1 is expressed only on early differentiating cells.
Additional information: Clone REA321 displays negligible binding to Fc receptors.

Alternative names

FUT4, CD15, ELFT, FCT3A, FUC-TIV, FUTIV, Lex

Technical specifications

  • Antigen: SSEA-1
  • Clone: REA321
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: FUT4, CD15, ELFT, FCT3A, FUC-TIV, FUTIV, Lex
  • Distribution of antigen: monocytes, eosinophils, neutrophils, ES and iPS cells, other
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE
  • Selected references

    1. Son, M. J. et al. (2009) SSEA-1 is an enrichment marker for tumor-initiating cells in human glioblastoma. Cell Stem Cell 4(5): 440-452
    2. Knowles, B. B. et al. (1982) Murine embryonic antigen (SSEA-1) is expressed on human cells and structurally related human blood group antigen I is expressed on mouse embryo. Dev. Biol. 93(1): 54-58
    3. Nudelman, E. et al. (1980) Monoclonal antibody directed to the stage-specific embryonic antigen (SSEA-1) reacts with branched glycosphingolipid similar in structure to Ii antigen. Biochem. Biophys. Res. Commun. 97(2): 443-451
  • Customer reports and application notes

REAfinity clone is tested against different known clones, in a blocking experiment, to identify whether they recognize completely overlapping (++), partially overlapping (+), or completely different epitopes (-). In a blocking experiment, cells are incubated with an excess of purified unconjugated REAfinity clone followed by staining with conjugated form of the other known clones. 

Other clonesOverlap in epitope recognition with REA321
MC480++
80H5+
HI98+
Product options: 2
130-117-801

Anti-SSEA-1-PE, human and mouse

for 30
tests
(1)
-
130-117-689

Anti-SSEA-1-PE, human and mouse

for 100
tests
(1)
-