Anti-Sca-1 antibodies, mouse

Anti-Sca-1 antibodies, mouse

Clone: D7 | Dilution: 1:10
Sca-1 (stem cell antigen-1) is a 18 kDa GPI-linked surface protein of the Ly-6 family (Ly-6A/E). The Anti-Sca-1 antibody clone D7 specifically recognizes both Ly-6E.1 and Ly-6A.2, which are gene products of the two Ly-6A/E alleles expressed in different mouse strains (Ly-6E.1: e.g. BALB/c, C3H, NZB; Ly6A.2: e.g. C57BL/6, SJL, 129, AKR).
Sca-1 is expressed on hematopoietic stem cells and progenitor cells in mouse bone marrow and one of the defining markers for so-called KSL (c-kit
+
Sca-1
+
Lin
-
) cells. Sca-1 in combination with CD105 (Endoglin) expression has been used to define long-term repopulating hematopoietic stem cells (LTR-HSCs) in mouse bone marrow
1,2
. In addition, Sca-1
+
bone marrow cells also contain the mesenchymal stem cell fraction present. Sca-1
+
cells have been shown to give rise to hepatocytes
in vivo3
and neural cells
in vitro
.
4
Furthermore, Sca-1 is expressed on stem cells in a variety of nonhematopoietic tissues, such as adult liver
5
, heart
6
, and prostate.
7

Alternative Names

LY6A, Ly-6A.2, Ly-6A/E, Ly-6E.1, TAP

Technical specifications

  • Antigen: Sca-1
  • Clone: D7
  • Isotype: rat IgG2aκ
  • Isotype control: Rat IgG2a – isotype control antibodies
  • Alternative names of antigen: LY6A, Ly-6A.2, Ly-6A/E, Ly-6E.1, TAP
  • Entrez Gene ID: 110454
  • Molecular mass of antigen [kDa]: 9
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, PE, APC, Biotin
Product options: 7

Product information

Size
Order no.
Price

Anti-Sca-1-FITC, mouse

  • D7
  • 1:10
  • For research use only
9 µg in 300 µL (1)
130-102-831
-

Anti-Sca-1-FITC, mouse

  • D7
  • 1:10
  • For research use only
30 µg in 1 mL (1)
130-102-297
-

Anti-Sca-1-PE, mouse

  • D7
  • 1:10
  • For research use only
9 µg in 300 µL (1)
130-102-832
-

Anti-Sca-1-APC, mouse

  • D7
  • 1:10
  • For research use only
9 µg in 300 µL (1)
130-102-833
-

Anti-Sca-1-APC, mouse

  • D7
  • 1:10
  • For research use only
30 µg in 1 mL (1)
130-102-343
-

Anti-Sca-1-Biotin, mouse

  • D7
  • 1:10
  • For research use only
9 µg in 300 µL (1)
130-101-995
-

Anti-Sca-1-Biotin, mouse

  • D7
  • 1:10
  • For research use only
30 µg in 1 mL (1)
130-101-885
-

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