Anti-ROR1 antibodies, human

Anti-ROR1 antibodies, human

Clone: 2A2 | Dilution: 1:50
The monoclonal antibody 2A2 reacts with human receptor tyrosine kinase-like orphan receptor 1 (ROR1). ROR1 has characteristics of an oncofetal gene and was recently identified to be overexpressed at the gene and protein levels in chronic lymphocytic leukemia (CLL) and mantle cell lymphoma (MCL). ROR1 expression has also been detected in ovarian cancer, renal cancer, melanoma, and lung adenocarcinoma, suggesting a general role of ROR1 in cancer genesis and/or maintenance. Moreover, ROR1 expression has been found in undifferentiated embryonic stem cells, in adipose tissue, at early stages of B cell development, but not in major adult tissues. Therefore, ROR1 is a potential therapeutic target and a biomarker to identify and enumerate malignant cells in CLL and MCL blood samples via flow cytometry.

Alternative Names

NTRKR1

Technical specifications

  • Antigen: ROR1
  • Clone: 2A2
  • Isotype: mouse IgG1κ
  • Isotype control: Mouse IgG1 – isotype control antibodies
  • Alternative names of antigen: NTRKR1
  • Entrez Gene ID: 4919
  • Molecular mass of antigen [kDa]: 101
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: VioBright FITC, PE, APC, Biotin, pure
  • Selected References

    1. Hudecek, M. et al. (2010) The B-cell tumor–associated antigen ROR1 can be targeted with T cells modified to express a ROR1-specific chimeric antigen receptor. Blood 116(22): 4532-4541
    2. Fukuda, T. et al. (2008) Antisera induced by infusions of autologous Ad-CD154-leukemia B cells identify ROR1 as an oncofetal antigen and receptor for Wnt5a. Proc. Natl. Acad. Sci. U.S.A. 105: 3047-3052
    3. Yamaguchi, T. et al. (2012) NKX2-1/TITF1/TTF-1-induced ROR1 is required to sustain EGFR survival signaling in lung adenocarcinoma. Cancer Cell 21(3): 348-361
    4. Baskar, S. et al. (2008) Unique cell surface expression of receptor tyrosine kinase ROR1 in human B-cell chronic lymphocytic leukemia. Clin. Cancer Res. 14(2): 396-404
    5. Daneshmanesh, A. H. et al. (2012) Monoclonal antibodies against ROR1 induce apoptosis of chronic lymphocytic leukemia (CLL) cells. Leukemia 26(6): 1348-1355
    6. Yang, J. et al. (2011) Therapeutic potential and challenges of targeting receptor tyrosine kinase ROR1 with monoclonal antibodies in B-cell malignancies. PLoS One 6(6): e21018
    7. Zhang, S. et al. (2012) ROR1 is expressed in human breast cancer and associated with enhanced tumor-cell growth. PLoS Biol. 7(3): e31127
    8. Baskar, S. et al. (2012) Targeting malignant B cells with an immnunotoxin against ROR1. mAbs 4(3): 349-361
  • Customer reports and application notes