Anti-Nestin antibodies, mouse and rat

Anti-Nestin antibodies, mouse and rat

Clone: REA575 | Dilution: 1:50
Clone REA575 recognizes the mouse and rat Nestin antigen, a type VI intermediate filament protein, mainly expressed in the developing central nervous system and skeletal muscles. In adult eye, Nestin is present in the optic nerve but also transiently expressed in the midembryonic mouse heart. Nestin plays a role in regulation of the assembly and disassembly of phosphorylated vimentin intermediate filaments during mitosis. It is involved in the trafficking and distribution of intermediate filament (IF) proteins and other cellular factors to daughter cells during progenitor cell division.
Additional information: Clone REA575 displays negligible binding to Fc receptors.

Alternative Names

NES, ESTM46

Technical specifications

  • Antigen: Nestin
  • Clone: REA575
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control antibodies
  • Alternative names of antigen: NES, ESTM46
  • Entrez Gene ID: 18008, 25491
  • Molecular mass of antigen [kDa]: 207, 209
  • Distribution of antigen: neural stem cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE
  • Selected References

    1. Kachinsky, A. M. et al. (1995) Intermediate filaments in cardiac myogenesis: nestin in the developing mouse heart. J. Histochem. Cytochem. 43(8): 843-847
    2. Park, D. et al. (2010) Nestin is required for the proper self-renewal of neural stem cells. Stem Cells 28(12): 2162-2171
    3. Yang, J. et al. (2000) Nestin expression during mouse eye and lens development. Mech. Dev. 94(1-2): 287-291