Anti-Microfold (M) Cells antibodies – functional grade, mouse

Anti-Microfold (M) Cells antibodies – functional grade, mouse

Clone: NKM 16-2-4 
Clone NKM 16-2-4 specifically binds (1,2)-fucose–containing carbohydrate moieties on murine microfold cells (M cells or membranous cells). Villous M cells are located in follicle-associated epithelium of Peyer’ s patches (PPs) and nasopharynx-associated lymphoid tissue (NALT). NKM 16-2-4 specifically binds to all subsets of M cells, but not epithelial cells or goblet cells located in PPs, NALT, and the intestinal villi in the gastrointestinal or respiratory tracts.
Microfold cells or membranous cells (M cells) have a high transcytotic activity and play an important role in the uptake of luminal antigens for induction of antigen-specific immune responses. This antibody (clone NKM 16-2-4) specifically binds to all subsets of M cells, but not epithelial cells or goblet cells located in Peyer's patches, nasopharynx-associated lymphoid tissue, and the intestinal villi in the gastrointestinal or respiratory tracts.

Applications

The Anti-Microfold (M) Cells pure – functional grade antibody is suited for functional assays, for example, antibody-mediated vaccination studies.

Technical specifications

  • Antigen: Microfold
  • Clone: NKM 16-2-4 
  • Isotype: rat IgG2c
  • Endotoxin level: Endotoxin levels have been tested and do not exceed 0.01 ng/µg of protein.
  • Product format: Antibodies are supplied in phosphate-buffered saline (PBS), pH 7.2. The product contains no preservative and is sterile filtered. Always handle under aseptic conditions.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: pure
  • Selected references

    1. Nochi, T. et al. (2007) A novel M cell-specific carbohydrate-targeted mucosal vaccine effectively induces antigen-specific immune responses. J. Exp. Med. 204: 2789-2796
    2. Terahara, K. et al. (2008) Comprehensive gene expression profiling of Peyer's patch M cells, villous M-like cells, and intestinal epithelial cells. J. Immunol. 180: 7840-846
    3. Kim, S-H. et al. (2010) The M cell-targeting ligand promotes antigen delivery and induces antigen-specific immune responses in mucosal vaccination. J. Immunol. 185: 5787-5795
    4. Kim, D-Y. et al. (2011) The airway antigen sampling system: respiratory M cells as an alternative gateway for inhaled antigens. J. Immunol. 186: 4253-426
Product options: 1
130-096-150

Anti-Microfold (M) Cell pure - functional grade, mouse

500 µg in 0.5 mL
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