Anti-MHC Class II (I-Ek) antibodies, mouse and rat

Anti-MHC Class II (I-Ek) antibodies, mouse and rat

Clone: REA510 | Dilution: 1:10
Clone REA510 recognizes the mouse MHC class II alloantigen I-Ek. It also recognizes the rat MHC class II antigen RT1D and cells from mice of the H-2
d
, H-2
p
, and H-2
r
haplotypes. Cells from mice of the H-2
b
, H-2
f
, H-2
g7
, H-2
q
, and H-2
s
haplotypes do not express the I-E antigen. MHC class II is expressed on antigen-presenting cells, such as dendritic cells, monocytes, macrophages, B cells in lymphoid and non-lymphoid tissue, thymic epithelial cells, and on subsets of hematopoietic progenitor cells in the bone marrow.
Additional information: Clone REA510 displays negligible binding to Fc receptors.

Alternative Names

RT1D

Technical specifications

  • Antigen: MHC Class II (I-Ek)
  • Clone: REA510
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control antibodies
  • Alternative names of antigen: RT1D
  • Entrez Gene ID: 14969, 294270
  • Molecular mass of antigen [kDa]: 27
  • Distribution of antigen: B cells, dendritic cells, hematopoietic stem cells, macrophages, monocytes, thymic epithelial cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, VioBright FITC, PE, APC, VioBlue, VioGreen, PE-Vio770, APC-Vio770, PerCP-Vio700, Biotin
  • Selected References

    1. Vestberg, M. et al. (1998) Limited polymorphism in the first domain of the rat MHC class II RT1-D molecule. Immunogenetics 48(5): 344-349
    2. Yelon, D. et al. (1999)
      Alterations in CD4-binding regions of the MHC class II molecule I-Ek do not impede CD4
      +
      T cell development.
      J. Immunol. 162(3): 1348-1358
    3. Kasson, P. M. et al. (2000) Kinetics of peptide binding to the class II MHC protein I-Ek. Biochemistry 39(5): 1048-1058
Product options: 20

Product information

Size
Order no.
Price

Anti-MHC Class II (I-Ek)-FITC, mouse and rat

  • REA510
  • 1:10
  • For research use only
9 µg in 300 µL (1)
130-107-872
-

Anti-MHC Class II (I-Ek)-FITC, mouse and rat

  • REA510
  • 1:10
  • For research use only
30 µg in 1 mL (1)
130-107-819
-

Anti-MHC Class II (I-Ek)-VioBright FITC, mouse and rat

  • REA510
  • 1:10
  • For research use only
9 µg in 300 µL (1)
130-107-878
-

Anti-MHC Class II (I-Ek)-VioBright FITC, mouse and rat

  • REA510
  • 1:10
  • For research use only
30 µg in 1 mL (1)
130-107-825
-

Anti-MHC Class II (I-Ek)-PE, mouse and rat

  • REA510
  • 1:10
  • For research use only
9 µg in 300 µL (1)
130-107-873
-

Anti-MHC Class II (I-Ek)-PE, mouse and rat

  • REA510
  • 1:10
  • For research use only
30 µg in 1 mL (1)
130-107-820
-

Anti-MHC Class II (I-Ek)-APC, mouse and rat

  • REA510
  • 1:10
  • For research use only
9 µg in 300 µL (1)
130-107-874
-

Anti-MHC Class II (I-Ek)-APC, mouse and rat

  • REA510
  • 1:10
  • For research use only
30 µg in 1 mL (1)
130-107-821
-

Anti-MHC Class II (I-Ek)-VioBlue, mouse and rat

  • REA510
  • 1:10
  • For research use only
9 µg in 300 µL (1)
130-107-871
-

Anti-MHC Class II (I-Ek)-VioBlue, mouse and rat

  • REA510
  • 1:10
  • For research use only
30 µg in 1 mL (1)
130-107-818
-

Anti-MHC Class II (I-Ek)-VioGreen, mouse and rat

  • REA510
  • 1:10
  • For research use only
9 µg in 300 µL (1)
130-107-870
-

Anti-MHC Class II (I-Ek)-VioGreen, mouse and rat

  • REA510
  • 1:10
  • For research use only
30 µg in 1 mL (1)
130-107-817
-

Anti-MHC Class II (I-Ek)-PE-Vio770, mouse and rat

  • REA510
  • 1:10
  • For research use only
9 µg in 300 µL (1)
130-107-875
-

Anti-MHC Class II (I-Ek)-PE-Vio770, mouse and rat

  • REA510
  • 1:10
  • For research use only
30 µg in 1 mL (1)
130-107-822
-

Anti-MHC Class II (I-Ek)-APC-Vio770, mouse and rat

  • REA510
  • 1:10
  • For research use only
9 µg in 300 µL (1)
130-107-876
-

Anti-MHC Class II (I-Ek)-APC-Vio770, mouse and rat

  • REA510
  • 1:10
  • For research use only
30 µg in 1 mL (1)
130-107-823
-

Anti-MHC Class II (I-Ek)-PerCP-Vio700, mouse and rat

  • REA510
  • 1:10
  • For research use only
9 µg in 300 µL (1)
130-107-877
-

Anti-MHC Class II (I-Ek)-PerCP-Vio700, mouse and rat

  • REA510
  • 1:10
  • For research use only
30 µg in 1 mL (1)
130-107-824
-

Anti-MHC Class II (I-Ek)-Biotin, mouse and rat

  • REA510
  • 1:10
  • For research use only
9 µg in 300 µL (1)
130-107-869
-

Anti-MHC Class II (I-Ek)-Biotin, mouse and rat

  • REA510
  • 1:10
  • For research use only
30 µg in 1 mL (1)
130-107-816
-