Anti-MHC Class II (I-Ab) antibodies, mouse

Anti-MHC Class II (I-Ab) antibodies, mouse

Clone: REA528 | Dilution: 1:50
Clone REA528 recognizes the mouse MHC class II alloantigen I-Ab of H-2b bearing mouse strains. It also cross-reacts with the H-2k and H-2u haplotypes, but reactivity to other haplotypes like, for example, d, f, g7, p, q, r, and s has not been observed. MHC class II is expressed on antigen-presenting cells, such as dendritic cells, monocytes, macrophages, B cells in lymphoid and non-lymphoid tissue, thymic epithelial cells, and on subsets of hematopoietic progenitor cells in the bone marrow.
Additional information: Clone REA528 displays negligible binding to Fc receptors.

Alternative Names

MHC class II IAb

Technical specifications

  • Antigen: MHC Class II (I-Ab)
  • Clone: REA528
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control antibodies
  • Alternative names of antigen: MHC class II IAb
  • Entrez Gene ID: 14961
  • Molecular mass of antigen [kDa]: 27
  • Distribution of antigen: B cells, dendritic cells, hematopoietic stem cells, macrophages, monocytes, thymic epithelial cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE
  • Selected References

    1. Cuenca, M. et al. (2016) Targeting of Ly9 (CD229) disrupts marginal zone and B1 B cell homeostasis and antibody responses. J. Immunol. 196: 726-737
    2. Gradehandt, G. et al. (1993) The I-Ab-restricted alloresponse of D10.G4.1 T cells is based on the recognition of an endogenous peptide. Immunology 78(4): 592-599
    3. Tobita, T. et al. (2003) A role for the P1 anchor residue in the thermal stability of MHC class II molecule I-Ab. Immunol. Lett. 85(1): 47-52
    4. Zhu, Y. et al. (2003) Crystal structure of MHC class II I-Ab in complex with a human CLIP peptide: prediction of an I-Ab peptide-binding motif. J. Mol. Biol. 326(4): 1157-1174

REAfinity clone is tested against different known clones, in a blocking experiment, to identify whether they recognize completely overlapping (++), partially overlapping (+), or completely different epitopes (-). In a blocking experiment, cells are incubated with an excess of purified unconjugated REAfinity clone followed by staining with conjugated form of the other known clones. 

Other clonesOverlap in epitope recognition with REA528
Product options: 2

Product information

Order no.

Anti-MHC Class II (I-Ab)-PE, mouse

  • REA528
  • 1:50
  • For research use only
30 µg in 200 µL (1)

Anti-MHC Class II (I-Ab)-PE, mouse

  • REA528
  • 1:50
  • For research use only
150 µg in 1 mL (1)