Anti-Ly-6G antibodies, mouse

Anti-Ly-6G antibodies, mouse

Clone: REA526 | Dilution: 1:10
Clone REA526 recognizes the mouse lymphocyte antigen 6G (Ly-6G) antigen, a 21–25 kDa glycosylphosphatidylinositol (GPI)-linked protein which is highly expressed on neutrophilic granulocytes. Expression greatly increases during granulocyte maturation. To a lower extent, Ly-6G is also expressed during the development of monocytes. In mouse bone marrow samples, Ly-6G is primarily detected on granulocytes, but not on lymphocytes or erythrocytes. Ligation of Ly-6G in animals with an underlying background inflammation (induced by TNF-α) leads to profound cardiovascular and respiratory responses, including severe breathing difficulties, leukocyte and platelet aggregation, and coagulation, leading to microvessel obstruction and subsequent death.
Additional information: Clone REA526 displays negligible binding to Fc receptors.

Alternative Names

Ly-6G.1, Ly6g, Gr-1

Technical specifications

  • Antigen: Ly-6G
  • Clone: REA526
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control antibodies
  • Alternative names of antigen: Ly-6G.1, Ly6g, Gr-1
  • Entrez Gene ID: 546644
  • Molecular mass of antigen [kDa]: 10
  • Distribution of antigen: granulocytes, macrophages, monocytes
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, PE, APC, PE-Vio770, Biotin

REAfinity clone is tested against different known clones, in a blocking experiment, to identify whether they recognize completely overlapping (++), partially overlapping (+), or completely different epitopes (-). In a blocking experiment, cells are incubated with an excess of purified unconjugated REAfinity clone followed by staining with conjugated form of the other known clones. 

Other clonesOverlap in epitope recognition with REA526
REA810-
1A8++
RB6-8C5-
  • Selected References

    1. Carr, K. D. et al. (2011) Specific depletion reveals a novel role for neutrophil-mediated protection in the liver during Listeria monocytogenes infection. Eur. J. Immunol. 41(9): 2666-2676
    2. Tanaka, Y. et al. (2012) Stimulation of Ly-6G on neutrophils in LPS-primed mice induces platelet-activating factor (PAF)-mediated anaphylaxis-like shock. J. Leukoc. Biol. 91(3): 485-494
    3. Fleming, T. J. et al. (1993) Characterization of two novel Ly-6 genes. Protein sequence and potential structural similarity to alpha-bungarotoxin and other neurotoxins. J. Immunol. 150(12): 5379-5390
Product options: 10

Product information

Size
Order no.
Price

Anti-Ly-6G-FITC, mouse

  • REA526
  • 1:10
  • For research use only
9 µg in 300 µL (1)
130-107-974
-

Anti-Ly-6G-FITC, mouse

  • REA526
  • 1:10
  • For research use only
30 µg in 1 mL (1)
130-107-912
-

Anti-Ly-6G-PE, mouse

  • REA526
  • 1:10
  • For research use only
9 µg in 300 µL (1)
130-107-975
-

Anti-Ly-6G-PE, mouse

  • REA526
  • 1:10
  • For research use only
30 µg in 1 mL (1)
130-107-913
-

Anti-Ly-6G-APC, mouse

  • REA526
  • 1:10
  • For research use only
9 µg in 300 µL (1)
130-107-976
-

Anti-Ly-6G-APC, mouse

  • REA526
  • 1:10
  • For research use only
30 µg in 1 mL (1)
130-107-914
-

Anti-Ly-6G-PE-Vio770, mouse

  • REA526
  • 1:10
  • For research use only
9 µg in 300 µL (1)
130-107-977
-

Anti-Ly-6G-PE-Vio770, mouse

  • REA526
  • 1:10
  • For research use only
30 µg in 1 mL (1)
130-107-915
-

Anti-Ly-6G-Biotin, mouse

  • REA526
  • 1:10
  • For research use only
9 µg in 300 µL (1)
130-107-973
-

Anti-Ly-6G-Biotin, mouse

  • REA526
  • 1:10
  • For research use only
30 µg in 1 mL (1)
130-107-911
-