Anti-Ly-49I antibodies, mouse

Anti-Ly-49I antibodies, mouse

Clone: REA1043 | Dilution: 1:50
Clone REA1043 recognizes the mouse Ly49I antigen, a single-pass type II C-type lectin-like membrane glycoprotein also known as Ly49 inhibitory receptor I. The Ly49 receptors are encoded by a family of highly polymorphic and polygenic genes within the mouse NK gene complex which includes genes that encode both inhibitory and activating Ly49 receptors. Ly49 receptors recognize MHC-I and MHC-I–like proteins on normal as well as on altered cells. They are described as being expressed on both the developing and mature NK cells. The inhibitory Ly49 receptors are involved in NK cell education, a process in which NK cells acquire function and tolerance toward cells that express “self-MHC-I.” On the other hand, the activating Ly49 receptors recognize altered cells expressing activating ligands. Ly49 receptors have been described on multiple NK cell subsets, such as uterine NK and memory NK cells, as well as NKT cells, dendritic cells, plasmacytoid dendritic cells, macrophages, neutrophils, and cells of the adaptive immune system, such as activated T cells and regulatory CD8
+
T cells. Ly49I is an inhibitory receptor capable of down-modulating proliferation, IFN-γ response, and cytotoxic activity in cells that express it.
Additional information: Clone REA1043 displays negligible binding to Fc receptors.

Alternative names

Ly-49 inhibitory receptor I

Technical specifications

  • Antigen: Ly-49I
  • Clone: REA1043
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control antibodies
  • Alternative names of antigen: Ly-49 inhibitory receptor I
  • Entrez Gene ID: 16640
  • Molecular mass of antigen [kDa]: 31
  • Distribution of antigen: NK cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, PE, APC, PE-Vio615, PE-Vio770, APC-Vio770, Biotin
  • Selected references

    1. Wong, S. et al. (1991) Ly-49 multigene family. New members of a superfamily of type II membrane proteins with lectin-like domains. J. Immunol. 147(4): 1417-1423
    2. Dimasi, N. et al. (2002) Crystal structure of the Ly49I natural killer cell receptor reveals variability in dimerization mode within the Ly49 family. J. Mol. Biol. 320(3): 573-585
    3. Tu, M. M. et al. (2014) Ly49 family receptors are required for cancer immunosurveillance mediated by natural killer cells. Cancer Res. 74(14): 3684-3694
Product options: 13
130-117-601

Anti-Ly-49I-FITC, mouse

30 µg in 200 µL (1)
-
130-117-452

Anti-Ly-49I-FITC, mouse

150 µg in 1 mL (1)
-
130-117-602

Anti-Ly-49I-PE, mouse

30 µg in 200 µL (1)
-
130-117-453

Anti-Ly-49I-PE, mouse

150 µg in 1 mL (1)
-
130-117-603

Anti-Ly-49I-APC, mouse

30 µg in 200 µL (1)
-
130-117-454

Anti-Ly-49I-APC, mouse

150 µg in 1 mL (1)
-
130-117-604

Anti-Ly-49I-PE-Vio615, mouse

30 µg in 200 µL (1)
-
130-117-455

Anti-Ly-49I-PE-Vio615, mouse

150 µg in 1 mL (1)
-
130-117-605

Anti-Ly-49I-PE-Vio770, mouse

30 µg in 200 µL (1)
-
130-117-456

Anti-Ly-49I-PE-Vio770, mouse

150 µg in 1 mL (1)
-
130-117-606

Anti-Ly-49I-APC-Vio770, mouse

30 µg in 200 µL (1)
-
130-117-608

Anti-Ly-49I-Biotin, mouse

30 µg in 200 µL (1)
-
130-117-459

Anti-Ly-49I-Biotin, mouse

150 µg in 1 mL (1)
-