Anti-Ly-49E/F antibodies, mouse

Anti-Ly-49E/F antibodies, mouse

Clone: REA218 | Dilution: 1:10
Clone REA218 recognizes the mouse killer cell lectin-like receptor 5 and 6 (Ly-49E and Ly-49F) antigens, which are type II transmembrane natural killer cell receptors belonging to the Ly-49 family. Members of the Ly-49 family are homodimeric C-type lectin receptors, recognizing MHC class I molecules as ligands and include activating and inhibitory receptor molecules. Ly-49E is expressed abundantly on fetal or immature NK cells and on certain γδ T cells, but at barely detectable frequencies on adult splenic NK cells. Different from other Ly-49 receptors, Ly-49E contains an immunoreceptor tyrosine-based inhibitory motif domain, a charged transmembrane residue and it is present in all mouse strains. Its failure to bind to a variety of soluble or cell bound MHC class I molecules indicates that its natural ligand is not an endogenous class I molecule. IL-2 and IL-15 selectively induce the
de novo
expression of Ly-49E on the majority of mature NK cells. By contrast, IL-4 blocks this IL-2 induced acquisition of Ly-49E. Ly-49F is expressed on subsets of NK cells, NK-T cells, and on a subpopulation of memory T cells in C57BL/6 mice, but not AKR/J, BALB/c, C3H/HeN, DBA/1, or SJL mice. IL-21 selectively down-regulates Ly-49F on mature NK cells.
Additional information: Clone REA218 displays negligible binding to Fc receptors.

Alternative Names

Klra5, Ly49e, Ly-49F, Klra6

Technical specifications

  • Antigen: Ly-49E/F
  • Clone: REA218
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control antibodies
  • Alternative names of antigen: Klra5, Ly49e, Ly-49F, Klra6
  • Entrez Gene ID: 16636, 16637
  • Molecular mass of antigen [kDa]: 31
  • Distribution of antigen: T cells, NK cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, PE, APC, VioBlue, PE-Vio770, APC-Vio770, PerCP-Vio700, Biotin
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