Anti-LGR5 antibodies, human

Anti-LGR5 antibodies, human

Clone: DA03-22H2.8 | Dilution: 1:50
Clone DA03-22H2.8 reacts with the human leucine-rich repeat-containing G-protein–coupled receptor 5 (LGR5) but not with its close homologues LGR4 or LGR6. LGR5 associates with Wnt-receptors and act as R-spondin receptor thereby playing a central role in the modulation of Wnt/β-catenin signaling in normal and neoplastic stem cells. Initially described as a highly specific marker for stem cells in the small intestine, colon, hair follicle, stomach, and during kidney development, LGR5 positive cells were also shown to be crucial during the development and progression of cancer. It was shown that LGR5 positive crypt stem cells are the cells-of-origin in intestinal cancer and that CSCs in human colorectal cancer can be identified and isolated based on LGR5 expression.

Alternative Names

Fex, GPR49, GPR67, GRP49, HG38

Technical specifications

  • Antigen: LGR5
  • Clone: DA03-22H2.8
  • Isotype: rat IgG2b
  • Isotype control: Rat IgG2b – isotype control antibodies
  • Alternative names of antigen: Fex, GPR49, GPR67, GRP49, HG38
  • Distribution of antigen: cancer stem cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE
  • Selected References

    1. Barker, N. et al. (2007) Identification of stem cells in small intestine and colon by marker gene Lgr5. Nature 449(7165): 1003-1007
    2. Barker, N. et al. (2009) Crypt stem cells as the cells-of-origin of intestinal cancer. Nature 457: 608-611
    3. de Lau, W. et al. (2011) Lgr5 homologues associate with Wnt receptors and mediate R-spondin signalling. Nature 476(7360): 293-297
    4. Barker, N. et al. (2010)
      Lgr5
      +
      stem cells drive self-renewal in the stomach and build long-lived gastric units
      in vitro
      .
      Cell Stem Cell 6(1): 25-36
    5. Carmon, K. S. et al. (2011) R-spondins function as ligands of the orphan receptors LGR4 and LGR5 to regulate Wnt/β-catenin signaling. Proc. Natl. Acad. Sci. U.S.A. 108: 11452-11457
    6. Dame, M. K. et al. (2014) Human colonic crypts in culture: segregation of immunochemical markers in normal versus adenoma-derived. Lab. Invest. 94(2): 222-234
    7. Jaks, V. et al. (2008) Lgr5 marks cycling, yet long-lived, hair follicle stem cells. Nat. Genet. 40: 1291-1299
    8. Kemper, K. et al. (2012) Monoclonal antibodies against lgr5 identify human colorectal cancer stem cells. Stem Cells 30(11): 2378-2386
    9. Barker, N. et al. (2012)
      LGR5
      +
      stem/progenitor cells contribute to nephron formation during kidney development.
      Cell Rep 2: 540-552
  • Scientific posters

Product options: 2

Product information

Size
Order no.
Price

Anti-LGR5-PE, human

  • DA03-22H2.8
  • 1:50
  • For research use only
for 30
tests
(1)
130-117-505
-

Anti-LGR5-PE, human

  • DA03-22H2.8
  • 1:50
  • For research use only
for 100
tests
(1)
130-117-355
-