Anti-IL-5 antibodies – functional grade, mouse

Anti-IL-5 antibodies – functional grade, mouse

Clone: TRFK-5
Interleukin 5 (IL-5) is pre-dominantly secreted by CD4
+
T cells. It is involved in a range of allergic reactions and mediates immune reactions against parasites. IL-5 also acts on other cell types such as B cells.
The Anti-IL-5 antibody has been designed for intracellular staining of IL-5–producing cells. Cells can be stimulated for IL-5-production, for example, by polyclonal stimulation with mitogens. For induction of IL-5 production by antigen-specific T cells, cells are restimulated with respective antigen. IL-5 can be accumulated in the cells by addition of secretion inhibitors like brefeldin A. After fixation and permeabilization of the cell sample, IL-5–producing cells can be stained intracellularly with Anti-IL-5 antibodies. Staining of surface markers allows simultaneous flow cytometric analysis of subsets and activation status of the IL-5–producing cells. Magnetically enriched cells can be stained intracellularly for IL-5 production directly on the MACS
®
Column. This procedure ensures higher sensitivity of detection and minimizes loss of cells during washing procedures. The protocol is useful for cytokine analysis of rare cells, for example, CD4
+
T cells in HIV patients, or other cell sources than peripheral blood mononuclear cells (PBMCs), e.g., bronchoalveolar lavages or synovial fluids.

Applications

This pure – functional grade antibody is suited for functional assays, for example, neutralization of cytokine activity.

Technical specifications

  • Antigen: IL-5
  • Clone: TRFK-5
  • Isotype: rat IgG1
  • Entrez Gene ID: 16191
  • Molecular mass of antigen [kDa]: 13
  • Endotoxin level: Endotoxin levels have been tested and do not exceed 0.01 ng/µg of protein.
  • Product format: Antibodies are supplied in phosphate-buffered saline (PBS), pH 7.2. The product contains no preservative and is sterile filtered. Always handle under aseptic conditions.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: pure
Product options: 1

Product information

Size
Order no.
Price

Anti-IL-5 pure - functional grade, mouse

  • TRFK-5
  • For research use only
500 µg in 0.5 mL
130-095-712
-

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