Anti-IL-4 antibodies, mouse

Anti-IL-4 antibodies, mouse

Clone: BVD4-1D11 | Dilution: 1:10
Interleukin-4 (IL-4) is predominantly secreted by activated CD4
+
memory and effector Tʜ2 cells, basophils, and mast cells. It induces and supports humoral immune responses for the neutralization of extracellular pathogens. The Tʜ2 types of immune mechanisms can also be involved in immunological disorders such as IgE-mediated allergy.
The Anti-IL‑4 antibody has been designed for intracellular staining of IL‑4–producing cells. Cells can be stimulated for IL‑4–production, e.g., by polyclonal stimulation with mitogens. For induction of IL‑4 production by antigen-specific T cells, cells are restimulated with respective antigen. IL‑4 can be accumulated in the cells by addition of secretion inhibitors like brefeldin A. After fixation and permeabilization of the cell sample, IL‑4–producing cells can be stained intracellularly with the Anti-IL‑4 antibody. Staining of surface markers allows simultaneous flow cytometric analysis of subsets and activation status of the IL‑4–producing cells. Magnetically enriched cells can be stained intracellularly for IL‑4 production directly on the MACS
®
Column. This procedure ensures higher sensitivity of detection and minimizes loss of cells during washing procedures for cytokine analysis of rare cells, e.g., CD4
+
T cells in HIV patients, or other cell sources than peripheral blood mononuclear cells (PBMCs), e.g., bronchoalveolar lavages, or synovial fluids.

Alternative Names

BSF-1

Technical specifications

  • Antigen: IL-4
  • Clone: BVD4-1D11
  • Isotype: rat IgG2bκ
  • Isotype control: Rat IgG2b – isotype control antibodies
  • Alternative names of antigen: BSF-1
  • Entrez Gene ID: 16189
  • Molecular mass of antigen [kDa]: 14
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE, APC, Vio515
Product options: 6

Product information

Size
Order no.
Price

Anti-IL-4-PE, mouse

  • BVD4-1D11
  • 1:10
  • For research use only
9 µg in 300 µL (1)
130-103-016
-

Anti-IL-4-PE, mouse

  • BVD4-1D11
  • 1:10
  • For research use only
30 µg in 1 mL (1)
130-102-435
-

Anti-IL-4-APC, mouse

  • BVD4-1D11
  • 1:10
  • For research use only
9 µg in 300 µL (1)
130-103-002
-

Anti-IL-4-APC, mouse

  • BVD4-1D11
  • 1:10
  • For research use only
30 µg in 1 mL (1)
130-102-398
-

Anti-IL-4-Vio515, mouse

  • BVD4-1D11
  • 1:10
  • For research use only
9 µg in 300 µL (1)
130-108-190
-

Anti-IL-4-Vio515, mouse

  • BVD4-1D11
  • 1:10
  • For research use only
30 µg in 1 mL (1)
130-108-167
-

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