Anti-IL-4 antibodies – functional grade, human

Anti-IL-4 antibodies – functional grade, human

Clone: 7A3-3
Interleukin-4 (IL-4) is predominantly secreted by activated CD4
+
memory and effector Tʜ2 cells, basophils, and mast cells. It induces and supports humoral immune responses for the neutralization of extracellular pathogens. The Tʜ2 types of immune mechanisms can also be involved in immunological disorders such as IgE-mediated allergy.
The Anti-IL‑4 antibody has been designed for intracellular staining of IL‑4–producing cells. Cells can be stimulated for IL‑4–production, e.g., by polyclonal stimulation with mitogens. For induction of IL‑4 production by antigen-specific T cells, cells are restimulated with respective antigen. IL‑4 can be accumulated in the cells by addition of secretion inhibitors like brefeldin A. After fixation and permeabilization of the cell sample, IL‑4–producing cells can be stained intracellularly with the Anti-IL‑4 antibody. Staining of surface markers allows simultaneous flow cytometric analysis of subsets and activation status of the IL‑4–producing cells. Magnetically enriched cells can be stained intracellularly for IL‑4 production directly on the MACS
®
Column. This procedure ensures higher sensitivity of detection and minimizes loss of cells during washing procedures for cytokine analysis of rare cells, e.g., CD4
+
T cells in HIV patients, or other cell sources than peripheral blood mononuclear cells (PBMCs), e.g., bronchoalveolar lavages, or synovial fluids.

Applications

The Anti-IL-4 pure – functional grade antibody is suited for functional assays, for example, neutralization of IL-4 activity,
in vitro
inhibition of Tʜ2 cell polarization and promotion of Tʜ1 cells, and Tʜ17 cell polarization.

Alternative Names

BCGF-1, BCGF1, BSF-1, BSF1

Technical specifications

  • Antigen: IL-4
  • Clone: 7A3-3
  • Isotype: mouse IgG1
  • Alternative names of antigen: BCGF-1, BCGF1, BSF-1, BSF1
  • Entrez Gene ID: 3565
  • Molecular mass of antigen [kDa]: 15
  • Cross-reactivity:
    rhesus monkey (
    Macaca mulatta
    )
    ,
    cynomolgus monkey (
    Macaca fascicularis
    )
  • Endotoxin level: Endotoxin levels have been tested and do not exceed 0.01 ng/µg of protein.
  • Product format: Antibodies are supplied in phosphate-buffered saline (PBS), pH 7.2. The product contains no preservative and is sterile filtered. Always handle under aseptic conditions.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: pure
Product options: 1

Product information

Size
Order no.
Price

Anti-IL-4 pure - functional grade, human

  • 7A3-3
  • For research use only
500 µg in 0.5 mL
130-095-753
-

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