Anti-IL-22 antibodies, human

Anti-IL-22 antibodies, human

Clone: REA466 | Dilution: 1:10
Clone REA466 recognizes the human interleukin-22 (IL-22) antigen, a secreted molecule which belongs to the IL-10 family of cytokines. IL-22 is produced by immune cells, including T helper cell subsets and innate lymphocytes, but acts only on non-hematopoietic stromal cells, in particular epithelial cells, keratinocytes, and hepatocytes. It plays an important role in host defense at mucosal surfaces as well as in tissue repair. Although IL-22 is beneficial to the host in many infectious and inflammatory disorders, depending on the target tissue it can be pathogenic due to its inherent pro-inflammatory properties, which are further enhanced when IL-22 is released together with other pro-inflammatory cytokines, in particular IL-17.
Additional information: Clone REA466 displays negligible binding to Fc receptors.

Alternative Names

IL-TIF, IL-D110, ILTIF, TIFIL-23, TIFa, zcyto18, IL-21

Technical specifications

  • Antigen: IL-22
  • Clone: REA466
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (I) antibodies
  • Alternative names of antigen: IL-TIF, IL-D110, ILTIF, TIFIL-23, TIFa, zcyto18, IL-21
  • Entrez Gene ID: 50616
  • Molecular mass of antigen [kDa]: 17
  • Distribution of antigen: T cells, T helper cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE, APC, Vio515, Vio667

Specificity

In order to compare the epitope specificity of REAfinity Clone REA466 with other known clones, a competition assay was performed. 
 Cells were incubated with an excess of purified unconjugated REAfinity Antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.
 

Other clonesOverlap in epitope recognition with REA466
2G12A41++
142928++
22URTI++
IL22JOP++
REA178++
REA307-
  • Selected References

    1. Dumoutier, L. et al. (2000) Human interleukin-10-related T cell-derived inducible factor: molecular cloning and functional characterization as an hepatocyte-stimulating factor. Proc. Natl. Acad. Sci. U.S.A. 97(18): 10144-10149
    2. Xie, M. H. et al. (2013) Interleukin (IL)-22, a novel human cytokine that signals through the interferon receptor-related proteins CRF2-4 and IL-22R. J. Biol. Chem. 275(40): 31335-31339
    3. Rutz, S. et al. (2013) IL-22, not simply a Tʜ17 cytokine. Immunol. Rev. 275(1): 116-132
Product options: 8
130-107-019

Anti-IL-22-PE, human

for 30
tests
(1)
-
130-106-958

Anti-IL-22-PE, human

for 100
tests
(1)
-
130-107-020

Anti-IL-22-APC, human

for 30
tests
(1)
-
130-106-959

Anti-IL-22-APC, human

for 100
tests
(1)
-
130-108-131

Anti-IL-22-Vio515, human

for 30
tests
(1)
-
130-108-096

Anti-IL-22-Vio515, human

for 100
tests
(1)
-
130-111-934

Anti-IL-22-Vio667, human

for 30
tests
(1)
-
130-111-768

Anti-IL-22-Vio667, human

for 100
tests
(1)
-