Anti-IL-17A antibodies, human

Anti-IL-17A antibodies, human

Clone: REA1063 | Dilution: 1:50
Clone REA1063 recognizes human interleukin 17A (IL-17A). IL-17A, also known as CTLA8, is a member of the IL-17 family (IL-17A–F), is a disulfide-linked homodimeric glycoprotein. IL-17A is produced by CD4
+
T helper (Tʜ) cells, a third T cell subset termed Tʜ17, which secrete also cytokines such as IL-17F and IL-22 and express the natural killer (NK) cell marker CD161. IL-17A secretion has also been described for other cell types, such as CD8
+
memory T cells. Furthermore, intracellular IL-17A has also been detected in eosinophils, neutrophils, and blood monocytes. Emerging data about Tʜ17 cells suggest that these cells are involved in the recruitment of neutrophils to control early stages of infections to a number of pathogens, such as extracellular bacteria and fungi. IL-17A and Tʜ17 cells have been shown to play an important role in immune-mediated diseases, such as rheumatoid arthritis, psoriasis, multiple sclerosis, asthma, inflammatory bowel diseases, and other immune-mediated inflammatory conditions.
Depending on the cytokine milieu present at time of the initial engagement, CD4
+
naive T cells can differentiate into various subsets (Tʜ1, Tʜ2, and Tʜ17). For the differentiation into Tʜ17 cells several cytokines have been described, including TGF-β, IL-1β, IL-6, IL-21, and IL-23. RORγt was identified as the master regulator gene for Tʜ17 cells.
Additional information: Clone REA1063 displays negligible binding to Fc receptors.

Alternative names

CTLA-8, IL-17

Technical specifications

  • Antigen: IL-17A
  • Clone: REA1063
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (I) antibodies
  • Alternative names of antigen: CTLA-8, IL-17
  • Entrez Gene ID: 3605
  • Molecular mass of antigen [kDa]: 15
  • Distribution of antigen: eosinophils, monocytes, neutrophils, T cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, PE, APC, PE-Vio615, PE-Vio770, APC-Vio770, Vio515

Specificity

In order to compare the epitope specificity of REAfinity Clone REA1063 with other known clones, a competition assay was performed. 
 Cells were incubated with an excess of purified unconjugated REAfinity Antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.
 

Other clonesOverlap in epitope recognition with REA1063
CZ8-23G1++
BL168++
N49-653++
eBio64DEC17-
  • Selected references

    1. Yao, Z. et al. (1995) Human IL-17: a novel cytokine derived from T cells. J. Immunol. 155: 5483-5486
    2. Kolls, J. K. and Linden, A. (2004) Interleukin-17 family members and inflammation. Immunity 21: 467-476
    3. Ivanov, I. I. et al. (2006)
      The orphan nuclear receptor RORγt directs the differentiation program of proinflammatory IL-17
      +
      T helper cells.
      Cell 126: 1121-1133
    4. Cosmi, L. et al. (2008)
      Human interleukin 17–producing cells originate from a CD161
      +
      CD4
      +
      T cell precursor.
      J. Exp. Med. 205: 1903-1916
    5. Manel, H. et al. (2008) The differentiation of human Tʜ-17 cells requires transforming growth factor-β and induction of the nuclear receptor RORγt. Nat. Immunol. 9: 641-649
    6. Tesmer, L. A. et al. (2008) Tʜ17 cells in human disease. Immunol. Rev. 223: 87-113
    7. Volpe, E. et al. (2008) A critical function for transforming growth factor-β, interleukin 23 and proinflammatory cytokines in driving and modulating human Tʜ-17 responses. Nat. Immunol. 9: 650-657
    8. Yang, L. et al. (2008) IL-21 and TGF-beta are required for differentiation of human Tʜ17 cells. Nature 454: 350-352
Product options: 14
130-118-396

Anti-IL-17A-FITC, human

for 30
tests
(1)
-
130-118-242

Anti-IL-17A-FITC, human

for 100
tests
(1)
-
130-118-397

Anti-IL-17A-PE, human

for 30
tests
(1)
-
130-118-243

Anti-IL-17A-PE, human

for 100
tests
(1)
-
130-118-398

Anti-IL-17A-APC, human

for 30
tests
(1)
-
130-118-244

Anti-IL-17A-APC, human

for 100
tests
(1)
-
130-118-401

Anti-IL-17A-PE-Vio615, human

for 30
tests
(1)
-
130-118-247

Anti-IL-17A-PE-Vio615, human

for 100
tests
(1)
-
130-118-402

Anti-IL-17A-PE-Vio770, human

for 30
tests
(1)
-
130-118-248

Anti-IL-17A-PE-Vio770, human

for 100
tests
(1)
-
130-118-403

Anti-IL-17A-APC-Vio770, human

for 30
tests
(1)
-
130-118-249

Anti-IL-17A-APC-Vio770, human

for 100
tests
(1)
-
130-118-404

Anti-IL-17A-Vio515, human

for 30
tests
(1)
-
130-118-250

Anti-IL-17A-Vio515, human

for 100
tests
(1)
-