Anti-IL-12 (p40/p70) antibodies – functional grade, human

Anti-IL-12 (p40/p70) antibodies – functional grade, human

Clone: C8.6
Interleukin 12 (IL-12) is a 70–75 kDa heterodimeric cytokine composed of a 40 kDa heavy chain and a 35 kDa light chain. It is produced mainly by monocytes and macrophages as well as dendritic cells, primarily in response to stimulation with bacterial or viral antigens. IL-12 has also been described to be produced by non-hematopoietic cells such as keratinocytes and epidermoid carcinomas. IL-12 displays various effects on both T and NK cells, playing an essential role in the interaction between innate and adaptive immunity, for example, it induces the production of IFN-γ alone or in synergy with IL-2, is a co-stimulator of both NK and T cell proliferation, and enhances cytolytic activity of both cell types. Moreover, IL-12 promotes the development of T helper 1 (Tʜ1)-specific immune responses by promoting the differentiation of CD4
+
cells towards a Tʜ1 phenotype while inhibiting the production of Tʜ2 cells.
Clone C8.6 reacts with both, the p40 chain and the p70 heterodimer of human, rhesus monkey, and cynomolgus monkey IL-12.

Applications

This pure – functional grade antibody is suited for functional assays, for example, neutralization of cytokine activity.

Alternative names

IL12B, CLMF, CLMF2, Il-12b, IMD28, IMD29, NKSF, NKSF2

Technical specifications

  • Antigen: IL-12 (p40/p70)
  • Clone: C8.6
  • Isotype: mouse IgG1
  • Alternative names of antigen: IL12B, CLMF, CLMF2, Il-12b, IMD28, IMD29, NKSF, NKSF2
  • Entrez Gene ID: 3593
  • Molecular mass of antigen [kDa]: 35
  • Cross-reactivity:
    rhesus monkey (
    Macaca mulatta
    )
    ,
    cynomolgus monkey (
    Macaca fascicularis
    )
  • Endotoxin level: Endotoxin levels have been tested and do not exceed 0.01 ng/µg of protein.
  • Product format: Antibodies are supplied in phosphate-buffered saline (PBS), pH 7.2. The product contains no preservative and is sterile filtered. Always handle under aseptic conditions.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: pure
  • Selected references

    1. D´Andrea, A. et al. (1992) Production of natural killer cell stimulatory factor (interleukin 12) by peripheral blood mononuclear cells. J. Exp. Med. 176: 1387-1398
    2. Trinchieri, G. et al. (2003) Interleukin-12 and the regulation of innate resistance and adaptive immunity. Nat. Rev. Immunol. 3(2): 133-146
Product options: 1
130-095-755

Anti-IL-12 (p40/p70) pure - functional grade, human

500 µg in 0.5 mL
-