Anti-IFN-γ antibodies – functional grade, human

Anti-IFN-γ antibodies – functional grade, human

Clone: 45-15
Interferon gamma (IFN-γ) is produced by cells that are involved in inflammatory immune responses. IFN-γ is predominantly released by memory and effector CD4
+
and CD8
+
T cells as well as by NK cells upon activation. Quantitative analysis of IFN-γ–producing cells can provide important information on the course of immune responses. Anti-IFN‑γ antibodies are designed for intracellular staining of IFN‑γ–producing cells. Cells can be stimulated for IFN-γ–production, e.g. by polyclonal stimulation with mitogens. For induction of IFN‑γ–production by antigen-specific T cells, cells are restimulated with respective antigen. IFN‑γ can be accumulated in the cells by addition of secretion inhibitors like brefeldin A. After fixation and permeabilization of the cell sample, IFN‑γ–producing cells can be stained intracellularly with Anti-IFN‑γ antibodies. Staining of surface markers allows simultaneous flow cytometric analysis of subsets and activation status of the IFN-γ–producing cells.

Applications

The Anti-IFN-γ pure – functional grade antibody is suited for functional assays, for example, neutralization of IFN-γ activity,
in vitro
inhibition of Tʜ1 cell polarization, and promotion of Tʜ2 cell and Tʜ17 cell polarization.

Alternative Names

IFNg, IFG, IFI

Technical specifications

  • Antigen: IFN-γ
  • Clone: 45-15
  • Isotype: mouse IgG1
  • Alternative names of antigen: IFNg, IFG, IFI
  • Entrez Gene ID: 3458
  • Molecular mass of antigen [kDa]: 16
  • Cross-reactivity:
    rhesus monkey (
    Macaca mulatta
    )
    ,
    cynomolgus monkey (
    Macaca fascicularis
    )
  • Endotoxin level: Endotoxin levels have been tested and do not exceed 0.01 ng/µg of protein.
  • Product format: Antibodies are supplied in phosphate-buffered saline (PBS), pH 7.2. The product contains no preservative and is sterile filtered. Always handle under aseptic conditions.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: pure
Product options: 1

Product information

Size
Order no.
Price

Anti-IFN-γ pure - functional grade, human

  • 45-15
  • For research use only
500 µg in 0.5 mL
130-095-743
-

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