Anti-HLA-DR, DP, DQ antibodies, human

Anti-HLA-DR, DP, DQ antibodies, human

Clone: REA332 | Dilution: 1:50
Clone REA332 recognizes all major histocompatibility class (MHC) class II HLA-DR, DP, and most DQ antigens. Expressed on the surface of most nucleated cells, MHC class II molecules play an important role in the immune system. They are essential in the defense against infection and are a main consideration in transplantation medicine. In addition to presenting antigenic peptides from predominantly extracellular sources to CD4
T cells, MHC class II molecules also mediate the thymic selection of T helper cells. MHC class II molecules consist of an α and β chain and are transported to endosomal-lysosomal compartments by the invariant chain. In humans, MHC class II molecules are encoded by three different loci, HLA-DR, -DQ, and -DP, which display 70% similarity to each other. Despite the essential function of MHC class II molecules in immune defense against pathogens, some alleles are frequently linked to immune diseases.
Additional information: Clone REA332 displays negligible binding to Fc receptors.

Alternative names


Technical specifications

  • Antigen: HLA-DR, DP, DQ
  • Clone: REA332
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: HLA-DPA1, HLA-DPB1, HLA-DQA1, HLA-DQA2, HLA-DQB1, HLA-DQB2, HLA-DQB3, HLA-DRA, HLA-DRB1
  • Entrez Gene ID: 3123, 3122, 3127, 3126, 3125, 16149, 3115, 3113, 3117, 3118, 3119, 3120, 3121
  • Cross-reactivity:
    chimpanzee (
    Pan troglodytes
    olive baboon (
    Papio anubis
    cynomolgus monkey (
    Macaca fascicularis
    common marmoset (
    Callithrix jacchus
  • Distribution of antigen: nucleated cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE


In order to compare the epitope specificity of REAfinity Clone REA332 with other known clones, a competition assay was performed. 
 Cells were incubated with an excess of purified unconjugated REAfinity Antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Other clonesOverlap in epitope recognition with REA332
  • Selected references

    1. Denzin, L. et al. (2017) Neutralizing Antibody Responses to Viral Infections Are Linked to the Non-classical MHC Class II Gene H2-Ob. Immunity 47(2)
    2. van Lith, M. et al. (2010) HLA-DP, HLA-DQ, and HLA-DR have different requirements for invariant chain and HLA-DM. J. Biol. Chem. 285(52): 40800-40808
    3. Franchini, G. et al. (2018) HIV vaccine candidate activation of hypoxia and the inflammasome in CD14+ monocytes is associated with a decreased risk of SIVmac251 acquisition. Nat. Med. 24(6): 847-856
    4. Jones, E. Y. et al. (2006) MHC class II proteins and disease: a structural perspective. Nat. Rev. Immunol. 6(4): 271-282
    5. Nishimura, Y. et al. (1998) Peptide-based molecular analyses of HLA class II-associated susceptibility to autoimmune diseases. Int. Rev. Immunol. 17(5–6): 229-262
    6. Hoppstädter, J. et al. (2015) M2 polarization enhances silica nanoparticle uptake by macrophages. Front Pharmacol 6
Product options: 2

Anti-HLA-DR, DP, DQ-PE, human

for 30

Anti-HLA-DR, DP, DQ-PE, human

for 100