Anti-His antibodies

Anti-His antibodies

Clone: GG11-8F3.5.1 | Dilution: 1:11
The monoclonal Anti-His antibody facilitates the fast and convenient detection of His-tagged fusion proteins, expressed in either prokaryotic or eukaryotic cells. The conjugated antibody allows fast and convenient analysis of His-tagged fusion proteins. Conjugation of the Anti-His antibody to HRP simplifies Western blot or ELISA analysis as the incubation with secondary antibodies becomes dispensable. Fluorochorme-conjugated Anti-His antibodies enable direct flow cytometry and fluorescent microscopy analyses, while indirect fluorescent labeling of His fusion protein expressing cells is possible with the biotin-conjugated antibody. After incubation the Anti-His-HRP antibody can be directly detected using commercially available chemiluminescent reagents.
The monoclonal Anti-His antibody specifically recognizes proteins that contain a polyhistidine tag at their N- and C-terminus. However, the Anti-His-HRP (C-term.) antibody shows higher sensitivity and specificity for C-terminal His-tagged proteins.

Technical specifications

  • Antigen: His
  • Clone: GG11-8F3.5.1
  • Isotype: mouse IgG1
  • Isotype control: Mouse IgG1 – isotype control antibodies
  • Molecular mass of antigen [kDa]: 1
  • Distribution of antigen: eukaryotic cells, prokaryotic cells
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, Biotin, HRP, HRP (C.-term.)
Product options: 6
130-098-808

Anti-His-FITC

for 30
tests
(1)
-
130-092-675

Anti-His-FITC

for 100
tests
(1)
-
130-099-423

Anti-His-Biotin

for 30
tests
(1)
-
130-092-692

Anti-His-Biotin

for 100
tests
(1)
-
130-092-785

Anti-His-HRP

100 µL
-
130-092-783

Anti-His-HRP (C-term.)

100 µL
-