Anti-Granzyme B antibodies, human, mouse, rat

Anti-Granzyme B antibodies, human, mouse, rat

Clone: REA226 | Dilution: 1:50
Clone REA226 recognizes granzyme B, a 32 kDa member of serine protease family, which is involved in cell lysis mediated by cytotoxic T lymphocytes (CTLs) and NK cells. Also known as granule enzymes, granzymes comprise majority of the mass of cytolytic granules in cytotoxic T lymphocytes (CTLs) and NK cells. Granzyme B is one of the five granzymes expressed by humans. NK cells and γδ T cells express granzymes constitutively, whereas expression in T lymphocytes is stimulation dependent. Main function attributed to granzymes is to indue apoptotic death of infected and transformed cells, where granzyme B enters into the target cell in a perforin dependent manner and via cation-independent mannose-6-phosphate receptor (CI-MPR). Granzyme B cleaves cellular substrates such as procaspase-3 and Bid, which then initiate pathways involved in apoptotic cell death. Granzyme B is suggested to be the only mammalian serine protease that prefers acidic side chains.
Additional information: Clone REA226 displays negligible binding to Fc receptors.

Alternative names

GZMB, CCPI, CGL-1, CSP-B, CTLA-1, CTSGL1, HLP, SECT, Granzyme 2

Technical specifications

  • Antigen: Granzyme B
  • Clone: REA226
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (I) antibodies
  • Alternative names of antigen: GZMB, CCPI, CGL-1, CSP-B, CTLA-1, CTSGL1, HLP, SECT, Granzyme 2
  • Distribution of antigen: T cells, NK cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, PE, APC

Specificity

In order to compare the epitope specificity of REAfinity Clone REA226 with other known clones, a competition assay was performed. 
 Cells were incubated with an excess of purified unconjugated REAfinity Antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.
 

Other clonesOverlap in epitope recognition with REA226
GB11++
QA16A02+
  • Selected references

    1. Grossman, W. J. et al. (2004) Differential expression of granzymes A and B in human cytotoxic lymphocyte subsets and T regulatory cells. Blood 104: 2840-2848
    2. Müllbacher, A. et al. (1999) Granzymes are the essential downstream effector molecules for the control of primary virus infections by cytolytic leukocytes. Proc. Natl. Acad. Sci. U.S.A. 96(13): 13950-13955
    3. Trapani, J. A. (2001) Granzymes: a family of lymphocyte granule serine proteases. Genome Biol. 2(12): 3014.1-3014.7
Product options: 6
130-118-480

Anti-Granzyme B-FITC, human, mouse, rat

for 30
tests
(1)
-
130-118-341

Anti-Granzyme B-FITC, human, mouse, rat

for 100
tests
(1)
-
130-116-654

Anti-Granzyme B-PE, human, mouse, rat

for 30
tests
(1)
-
130-116-486

Anti-Granzyme B-PE, human, mouse, rat

for 100
tests
(1)
-
130-120-773

Anti-Granzyme B-APC, human, mouse, rat

for 30
tests
(1)
-
130-120-703

Anti-Granzyme B-APC, human, mouse, rat

for 100
tests
(1)
-