Anti-Gr-1 antibodies, mouse

Anti-Gr-1 antibodies, mouse

Clone: REA810 | Dilution: 1:50
Clone REA810 recognizes the mouse granulocyte-differentiation antigen-1 (Gr-1) antigen, also known as Ly-6G, a 21–25 kDa, GPI-anchored cell surface protein. Cross-reactivity of Gr-1 with Ly-6C was not detected on hematopoietic cells that express Ly-6C and are negative for Ly-6G. Gr-1 is expressed on mature granulocytes in bone marrow and peripheral tissues. The Anti-Gr-1 antibody also stains monocytes transiently during their differentiation in bone marrow and at low levels plasmacytoid dendritic cells in lymphoid tissues.
Additional information: Clone REA810 displays negligible binding to Fc receptors.

Alternative Names

Ly-6G, Ly-6G.1

Technical specifications

  • Antigen: Gr-1
  • Clone: REA810
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control antibodies
  • Alternative names of antigen: Ly-6G, Ly-6G.1
  • Entrez Gene ID: 546644
  • Molecular mass of antigen [kDa]: 10
  • Distribution of antigen: granulocytes, monocytes, bone marrow
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, PE, APC, VioBlue, VioGreen, PE-Vio615, PE-Vio770, APC-Vio770, PerCP-Vio700

REAfinity clone is tested against different known clones, in a blocking experiment, to identify whether they recognize completely overlapping (++), partially overlapping (+), or completely different epitopes (-). In a blocking experiment, cells are incubated with an excess of purified unconjugated REAfinity clone followed by staining with conjugated form of the other known clones. 

Other clonesOverlap in epitope recognition with REA810
REA526++
1A8++
RB6-8C5++
  • Selected References

    1. Fleming, T. J. et al. (1993) Characterization of two novel Ly-6 genes. Protein sequence and potential structural similarity to alpha-bungarotoxin and other neurotoxins. J. Immunol. 150(12): 5379-5390
    2. Fleming, T. J. et al. (1993) Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. RB6-8C5 mAb to granulocyte-differentiation antigen (Gr-1) detects members of the Ly-6 family. J. Immunol. 151: 2399-2408
    3. Nagendra, S. and Schlueter, A. J. (2004) Absence of cross-reactivity between murine Ly-6C and Ly-6G. Cytometry A 58: 195-200
Product options: 17

Product information

Size
Order no.
Price

Anti-Gr-1-FITC, mouse

  • REA810
  • 1:50
  • For research use only
30 µg in 200 µL (1)
130-112-311
-

Anti-Gr-1-FITC, mouse

  • REA810
  • 1:50
  • For research use only
150 µg in 1 mL (1)
130-112-153
-

Anti-Gr-1-PE, mouse

  • REA810
  • 1:50
  • For research use only
30 µg in 200 µL (1)
130-112-306
-

Anti-Gr-1-PE, mouse

  • REA810
  • 1:50
  • For research use only
150 µg in 1 mL (1)
130-112-148
-

Anti-Gr-1-APC, mouse

  • REA810
  • 1:50
  • For research use only
30 µg in 200 µL (1)
130-112-307
-

Anti-Gr-1-APC, mouse

  • REA810
  • 1:50
  • For research use only
150 µg in 1 mL (1)
130-112-149
-

Anti-Gr-1-VioBlue, mouse

  • REA810
  • 1:50
  • For research use only
30 µg in 200 µL (1)
130-112-302
-

Anti-Gr-1-VioBlue, mouse

  • REA810
  • 1:50
  • For research use only
150 µg in 1 mL (1)
130-112-144
-

Anti-Gr-1-VioGreen, mouse

  • REA810
  • 1:50
  • For research use only
30 µg in 200 µL (1)
130-112-303
-

Anti-Gr-1-PE-Vio615, mouse

  • REA810
  • 1:50
  • For research use only
30 µg in 200 µL (1)
130-112-304
-

Anti-Gr-1-PE-Vio615, mouse

  • REA810
  • 1:50
  • For research use only
150 µg in 1 mL (1)
130-112-146
-

Anti-Gr-1-PE-Vio770, mouse

  • REA810
  • 1:50
  • For research use only
30 µg in 200 µL (1)
130-112-308
-

Anti-Gr-1-PE-Vio770, mouse

  • REA810
  • 1:50
  • For research use only
150 µg in 1 mL (1)
130-112-150
-

Anti-Gr-1-APC-Vio770, mouse

  • REA810
  • 1:50
  • For research use only
30 µg in 200 µL (1)
130-112-309
-

Anti-Gr-1-APC-Vio770, mouse

  • REA810
  • 1:50
  • For research use only
150 µg in 1 mL (1)
130-112-151
-

Anti-Gr-1-PerCP-Vio700, mouse

  • REA810
  • 1:50
  • For research use only
30 µg in 200 µL (1)
130-112-310
-

Anti-Gr-1-PerCP-Vio700, mouse

  • REA810
  • 1:50
  • For research use only
150 µg in 1 mL (1)
130-112-152
-