Anti-Feeder Cells antibodies, mouse

Anti-Feeder Cells antibodies, mouse

Clone: mEF-SK4 | Dilution: 1:10
The most prevalent cultivation method for maintenance of mouse and human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells is co-cultivation on primary mouse embryonic fibroblast (mEF) feeder cell layers. Feeder cell layers of NIH3T3 fibroblasts or stromal derived OP9 cells are also frequently used in co-culture systems: NIH3T3 fibroblasts support primary keratinocyte cultures, OP9 cells are used for hematopoietic induction of ES cells.

Technical specifications

  • Antigen: Feeder Cells
  • Clone: mEF-SK4
  • Isotype: rat IgG1
  • Isotype control: Rat IgG1 – isotype control antibodies
  • Distribution of antigen: mouse embryonic fibroblast cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE, APC, Biotin
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