Anti-EGF Receptor antibodies, human

Anti-EGF Receptor antibodies, human

Clone: REA688 | Dilution: 1:50
Clone RE688 recognizes the human epidermal growth factor receptor (EGFR) antigen. EGFR is a single-pass type I membrane protein which is also known as receptor tyrosine-protein kinase ErbB-1 or HER1. It is a critical regulator of many normal cellular processes, including cell growth, differentiation, survival, and migration. The EGFR also is implicated in pathological processes of cellular transformation and oncogenesis due to its overexpression in many types of cancers and its ability to induce morphological transformation of cultured cells and tumor formation in nude mice. Many different signaling pathways have been discovered to mediate the effects of EGF, the most notable being Ras-mitogen-activated protein kinase (MAPK) and the phosphatidylinositol 3-kinase pathways. In these pathways EGF binds to the EGFR to induce dimerization, catalytic activation, and autophosphorylation of tyrosines in the C-terminal tail of the EGFR. These phosphorylated tyrosines provide docking sites for adapter proteins such as Grb2, Shc, and Gab2 to link the receptor to the Ras-MAPK, as well as phosphatidylinositol 3-kinase pathways.
Additional information: Clone REA688 displays negligible binding to Fc receptors.

Technical specifications

  • Antigen: EGF Receptor
  • Clone: REA688
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Entrez Gene ID: 1956
  • Molecular mass of antigen [kDa]: 132
  • Distribution of antigen: kidney, prostate, other
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: VioBright FITC, PE, APC, Biotin, pure
  • Selected references

    1. Ullrich, A. et al. (1984) Human epidermal growth factor receptor cDNA sequence and aberrant expression of the amplified gene in A431 epidermoid carcinoma cells. Nature 309(5967): 418-425
    2. Bell, G. I. et al. (1986)
      Human epidermal growth factor precursor: cDNA sequence, expression
      in vitro
      and gene organization.
      Nucleic Acids Res. 14(21): 8427-8446
    3. Heisermann, G. J. et al. (1988)
      Epidermal growth factor receptor threonine and serine residues phosphorylated
      in vivo
      .
      J. Biol. Chem. 263(26): 13152-13158
Product options: 9
130-110-588

Anti-EGF Receptor-VioBright FITC, human

for 30
tests
(1)
-
130-110-530

Anti-EGF Receptor-VioBright FITC, human

for 100
tests
(1)
-
130-110-586

Anti-EGF Receptor-PE, human

for 30
tests
(1)
-
130-110-528

Anti-EGF Receptor-PE, human

for 100
tests
(1)
-
130-110-587

Anti-EGF Receptor-APC, human

for 30
tests
(1)
-
130-110-529

Anti-EGF Receptor-APC, human

for 100
tests
(1)
-
130-110-585

Anti-EGF Receptor-Biotin, human

for 30
tests
(1)
-
130-110-527

Anti-EGF Receptor-Biotin, human

for 100
tests
(1)
-
130-110-227

Anti-EGF Receptor pure, human

100 µg in 1 mL
-