StraightFrom™ Whole Blood CD8 MicroBeads, human

StraightFrom™ Whole Blood CD8 MicroBeads, human

StraightFrom™ Whole Blood CD8 MicroBeads were developed for rapid positive selection of CD8
+
cells directly from whole blood or bone marrow. No sample preparation is required, including density gradient centrifugation or erythrocyte lysis.

Background information

In whole blood the CD8 antigen is expressed on human cytotoxic T cells and on a subset of CD16
+
NK cells. The CD8 antigen acts as an accessory molecule in the recognition of MHC class I/peptide complexes by the TCR heterodimer on CD8
+
cytotoxic T cells.

Detailed separation procedure

Anticoagulated cell samples of 0.25–15 mL are labeled with StraightFrom™ Whole Blood CD8 MicroBeads to be subsequently separated with the autoMACS
®
Pro Separator, the MultiMACS™ Cell24 Separator Plus, or using the Whole Blood Column Kit.

Downstream applications

StraightFrom™ Whole Blood MicroBeads allow the fast isolation of CD8
+
cells directly from whole blood or bone marrow, thus minimizing hands-on time and maximizing yield of target cells. StraightFrom Whole Blood MicroBeads in combination with the autoMACS
®
Pro Separators allow standardization of the cell separation procedure and safe handling of hazardous samples
1
. The purified CD8
+
cells are well-suited for further flow cytometric, functional, or molecular analysis including lineage-specific chimerism analysis after allogeneic stem cell transplantation
2,3
.

Columns

autoMACS
®
Columns or Whole Blood Columns.
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