StraightFrom™ Whole Blood CD15 MicroBeads, human

StraightFrom™ Whole Blood CD15 MicroBeads, human

StraightFrom™ Whole Blood CD15 MicroBeads have been developed for rapid positive selection of CD15
+
cells directly from whole blood or bone marrow. No sample preparation is required, no density gradient centrifugation or erythrocyte lysis.

Background information

StraightFrom™ Whole Blood CD15 MicroBeads recognize the carbohydrate structure 3-fucosyl-N-acetyllactosamine (3-FAL), also designated Lewis X. The CD15 antigen is expressed on neutrophils and eosinophils but not on basophils and lymphocytes.

Detailed separation procedure

Anticoagulated cell samples of 0.25–15 mL are labeled with StraightFrom™ Whole Blood CD15 MicroBeads to be subsequently separated with the autoMACS® Pro Separator, the MultiMACS™ Cell24 Separator Plus, or using the Whole Blood Column Kit.

Downstream applications

StraightFrom™ Whole Blood MicroBeads allow the fast isolation of CD15
+
cells directly from whole blood or bone marrow, thus minimizing hands-on time and maximizing yield of target cells. StraightFrom Whole Blood MicroBeads in combination with the autoMACS® Separators allow standardization of the cell separation procedure and safe handling of hazardous samples. The purified CD15
+
cells are well-suited for further flow cytometric, functional, or molecular analysis including lineage-specific chimerism analysis after allogeneic stem cell transplantation
1,2,3
.

Columns

autoMACS
®
Columns or Whole Blood Columns.
Video

How to isolate immune cells directly from blood

Follow this fast and easy protocol for magnetic isolation of immune cells directly from blood or blood products without the need for density gradient centrifugation.

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Product information

Size
Order no.
Price

StraightFrom Whole Blood CD15 MicroBeads, human

  • For research use only
for 40
mL whole blood
130-091-058
-

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