StraightFrom™ LRSC CD8 MicroBead Kit, human

StraightFrom™ LRSC CD8 MicroBead Kit, human

The StraightFrom™ MicroBead Kit was developed for the rapid positive selection of CD8
+
cells directly from one LRSC using the MultiMACS™ Cell24 Separator Plus for semi-automated separation or a MACS
®
Separator for manual separation. No sample preparation is required, including density gradient centrifugation, erythrocyte lysis, wash steps, or cell count. LS Columns and Whole Blood Column Elution Buffer are included in the kit to allow direct processing of an entire LRSC.

Background information

In blood the CD8 antigen is expressed on human cytotoxic T cells and on a subset of CD16
+
NK cells. The CD8 antigen acts as an accessory molecule in the recognition of MHC class I/peptide complexes by the TCR heterodimer on CD8
+
cytotoxic T cells.

Detailed separation procedure

One entire LRSC sample is labeled with StraightFrom™ LRSC CD8 MicroBeads and loaded onto LS Columns, which are placed in the magnetic field of a MultiMACS™ Cell24 Separator Plus or a MACS
®
Separator. After removing the column from the magnetic field, the magnetically retained CD4
+
T cells can be eluted as the positively selected cell fraction.

Downstream applications

The purified CD8
+
cells are well suited for further flow cytometric, functional, or molecular analysis.
Product options: 1

Product information

Size
Order no.
Price

StraightFrom LRSC CD8 MicroBead Kit, human

  • For research use only
for 1 LRSC
130-117-027
-

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