StraightFrom™ Buffy Coat CD8 MicroBead Kit, human

StraightFrom™ Buffy Coat CD8 MicroBead Kit, human

The StraightFrom™ Buffy Coat CD8 MicroBead Kit has been developed for the rapid positive selection of CD8
+
cells directly from one buffy coat using the MultiMACS™ Cell24 Separator Plus for semi-automated separation or a MACS
®
Separator for manual separation. No sample preparation is required, including density gradient centrifugation, erythrocyte lysis, wash steps, or cell count. Whole Blood Columns and Whole Blood Column Elution Buffer are included in the kit to allow direct processing of an entire buffy coat.

Background information

In blood the CD8 antigen is expressed on human cytotoxic T cells and on a subset of CD16
+
NK cells. The CD8 antigen acts as an accessory molecule in the recognition of MHC class I/peptide complexes by the TCR heterodimer on CD8
+
cytotoxic T cells.

Detailed separation procedure

One entire buffy coat is labeled with StraightFrom™ Buffy Coat CD8 MicroBeads and loaded onto Whole Blood Columns, which are placed in the magnetic field of a MultiMACS™ Cell24 Separator Plus or a MACS
®
Separator. After removing the column from the magnetic field, the magnetically retained CD8
+
cells can be eluted as the positively selected cell fraction.

Downstream applications

The purified CD8
+
cells are well-suited for further flow cytometric, functional, or molecular analysis.
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