StraightFrom™ Buffy Coat CD4 MicroBead Kit, human

StraightFrom™ Buffy Coat CD4 MicroBead Kit, human

The StraightFrom™ Buffy Coat CD4 MicroBead Kit has been developed for the rapid positive selection of CD4
+
cells directly from one buffy coat using the MultiMACS™ Cell24 Separator Plus for semi-automated separation or a MACS
®
Separator for manual separation. No sample preparation is required, including density gradient centrifugation, erythrocyte lysis, wash steps, or cell count. Whole Blood Columns and Whole Blood Column Elution Buffer are included in the kit to allow direct processing of an entire buffy coat.

Background information

The CD4 antigen is expressed on T helper cells and at a lower level on monocytes and dendritic cells. The CD4 antigen is an accessory molecule in the recognition of MHC class II/peptide complexes by the TCR heterodimer on CD4
+
T helper cells.

Detailed separation procedure

One entire buffy coat is labeled with StraightFrom™ Buffy Coat CD4 MicroBeads and loaded onto Whole Blood Columns, which are placed in the magnetic field of a MultiMACS™ Cell24 Separator Plus or a MACS
®
Separator. After removing the column from the magnetic field, the magnetically retained CD4
+
cells can be eluted as the positively selected cell fraction.

Downstream applications

The purified CD4
+
cells are well suited for further flow cytometric, functional, or molecular analysis.
Product options: 1

Product information

Size
Order no.
Price

StraightFrom Buffy Coat CD4 MicroBead Kit, human

  • For research use only
for 1
buffy coat
130-114-980
-

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