StraightFrom® Whole Blood CD3 MicroBeads, human

StraightFrom
®
Whole Blood CD3 MicroBeads
, human

StraightFrom
®
Whole Blood CD3 MicroBeads were developed for rapid positive selection of CD3
+
cells directly from whole blood or bone marrow. No sample preparation is required, including density gradient centrifugation or erythrocyte lysis.

Background information

StraightFrom
®
Whole Blood CD3 MicroBeads recognize the CD3 antigen which is associated with the T cell receptor (TCR) heterodimer. CD3 is expressed on all T cells, constituting 15–20% of human peripheral blood leukocytes. StraightFrom Whole Blood MicroBeads allow the fast isolation of CD3
+
cells directly from whole blood or bone marrow, thus minimizing hands-on time and maximizing yield of target cells. StraightFrom Whole Blood MicroBeads in combination with the autoMACS
®
Pro Separator allow standardization of the cell separation procedure and safe handling of hazardous samples.

Detailed separation procedure

Anticoagulated cell samples of 0.25–15 mL are labeled with StraightFrom
®
Whole Blood CD3 MicroBeads to be subsequently separated with the autoMACS
®
Pro Separator, the MultiMACS™ Cell24 Separator Plus, or using the Whole Blood Column Kit.

Downstream applications

The purified CD3
+
cells are well-suited for further flow cytometric, functional, or molecular analysis including lineage-specific chimerism analysis after allogeneic stem cell transplantation
1,2
.

Columns

autoMACS
®
Columns or Whole Blood Columns.
Video

How to isolate immune cells directly from blood

For more information, visit https://bit.ly/2RJiJUd Follow this fast and easy protocol for magnetic isolation of immune cells directly from blood or blood products without the need for density gradient centrifugation.

Product options: 1
130-090-874

StraightFrom Whole Blood CD3 MicroBeads, human

for 40
mL whole blood
-