StraightFrom® LRSC CD3 MicroBead Kit, human

StraightFrom
®
LRSC CD3 MicroBead Kit
, human

The StraightFrom
®
MicroBead Kit was developed for the rapid positive selection of CD3
+
T cells directly from one LRSC using the MultiMACS™ Cell24 Separator Plus for semi-automated separation or a MACS
®
Separator for manual separation. No sample preparation is required, including density gradient centrifugation, erythrocyte lysis, wash steps, or cell count. LS Columns and Whole Blood Column Elution Buffer are included in the kit to allow direct processing of an entire LRSC.

Background information

The CD3 antigen is associated with the T cell receptor (TCR) heterodimer. CD3 is expressed on all T cells, constituting 15–20% of human peripheral blood leukocytes.

Detailed separation procedure

One entire LRSC sample is labeled with StraightFrom
®
LRSC CD3 MicroBeads and loaded onto LS Columns, which are placed in the magnetic field of a MultiMACS™ Cell24 Separator Plus or a MACS
®
Separator. After removing the column from the magnetic field, the magnetically retained CD3
+
T cells can be eluted as the positively selected cell fraction.

Downstream applications

The purified CD3
+
cells are well suited for further flow cytometric, functional, or molecular analysis.
Product options: 1

Product information

Size
Order no.
Price

StraightFrom LRSC CD3 MicroBead Kit, human

  • For research use only
for 1 LRSC
130-121-309
-

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