StraightFrom® Leukopak® CD4 MicroBead Kit, human

StraightFrom
®
Leukopak
®
CD4 MicroBead Kit
, human

The StraightFrom
®
Leukopak
®
MicroBead Kit was developed for the rapid positive selection of CD4
+
T cells directly from Leukopak
®
using the MultiMACS™ Cell24 Separator Plus for semi-automated separation. No sample preparation is required, including density gradient centrifugation, erythrocyte lysis, or cell count. Multi-24 Column Blocks and Whole Blood Column Elution Buffer are included in the kit to allow direct processing of a 1/2 Leukopak
®
.

Background information

The CD4 antigen is expressed on T helper cells and at a lower level on monocytes and dendritic cells. The CD4 antigen is an accessory molecule in the recognition of MHC class II/peptide complexes by the TCR heterodimer on CD4
+
T helper cells.

Detailed separation procedure

A Leukopak
®
sample is labeled with StraightFrom
®
Leukopak
®
CD4 MicroBeads and loaded onto Multi-24 Column Block, which is placed in the magnetic field of a MultiMACS™ Cell24 Separator Plus. After removing the Column Block from the magnetic field, the magnetically retained CD4
+
T cells can be eluted as the positively selected cell fraction.

Downstream applications

The purified CD4
+
T cells are well suited for further flow cytometric, functional, or molecular analysis.

Columns

Multi-24 Column Block
Product options: 1

Product information

Size
Order no.
Price

StraightFrom
®
Leukopak
®
CD4 MicroBead Kit
, human

  • For research use only
for 0.5
Leukopak
®
130-117-022
-

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