StraightFrom® Leukopak® CD19 MicroBead Kit, human

StraightFrom
®
Leukopak
®
CD19 MicroBead Kit
, human

The StraightFrom
®
Leukopak
®
MicroBead Kit was developed for the rapid positive selection of CD19
+
cells directly from Leukopak
®
using the MultiMACS™ Cell24 Separator Plus for semi-automated separation. No sample preparation is required, including density gradient centrifugation, erythrocyte lysis, or cell count. Multi-24 Column Block and Whole Blood Column Elution Buffer are included in the kit to allow direct processing of a 1/2 Leukopak
®
.

Background information

The CD19 antigen is expressed from the early pro–B cell to the B cell blast stage. It is also present on most malignant B cells. CD19 expression is down-regulated upon terminal differentiation of B cells to plasma cells.

Detailed separation procedure

A Leukopak
®
sample is labeled with StraightFrom
®
Leukopak
®
CD19 MicroBeads and loaded onto Multi-24 Column Block, which is placed in the magnetic field of a MultiMACS™ Cell24 Separator Plus. After removing the column from the magnetic field, the magnetically retained CD19
+
cells can be eluted as the positively selected cell fraction.

Downstream applications

The purified CD19
+
cells are well suited for further flow cytometric, functional, or molecular analysis.

Columns

Multi-24 Column Block
Product options: 1

Product information

Size
Order no.
Price

StraightFrom Leukopak CD19 MicroBead Kit, human

  • For research use only
for 0.5
Leukopak
130-117-021
-

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