StraightFrom® Leukopak CD19 MicroBead Kit, human

Leukopak CD19 MicroBead Kit
, human

The StraightFrom
Leukopak MicroBead Kit was developed for the rapid positive selection of CD19
cells directly from Leukopak using the MultiMACS™ Cell24 Separator Plus for semi-automated separation. No sample preparation is required, including density gradient centrifugation, erythrocyte lysis, or cell count. Multi-24 Column Block and Whole Blood Column Elution Buffer are included in the kit to allow direct processing of a 1/2 Leukopak.

Background information

The CD19 antigen is expressed from the early pro–B cell to the B cell blast stage. It is also present on most malignant B cells. CD19 expression is down-regulated upon terminal differentiation of B cells to plasma cells.

Detailed separation procedure

A Leukopak sample is labeled with StraightFrom
Leukopak CD19 MicroBeads and loaded onto Multi-24 Column Block, which is placed in the magnetic field of a MultiMACS™ Cell24 Separator Plus. After removing the column from the magnetic field, the magnetically retained CD19
cells can be eluted as the positively selected cell fraction.

Downstream applications

The purified CD19
cells are well suited for further flow cytometric, functional, or molecular analysis.


Multi-24 Column Block
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Product information

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